Optimization of PCR Amplification and Sequence Alignment of Pholas orientalis Internal Transcribed Spacers (ITS) for Possible Determination of Origin-Specific Restriction Fragment Lenght Polymorphisms (RFLPs)

Optimization of PCR Amplification and Sequence Alignment of Pholas orientalis Internal Transcribed Spacers (ITS) for Possible Determination of Origin-Specific Restriction Fragment Lenght Polymorphisms (RFLPs)

Six PCR amplification protocols of the internal transcribed spacers (ITS) for bivalves, which utilized the primers designed by Heath, et al. (1995), were evaluated in this study. However, only that of Breusing (2012) yielded desired brands. This protocol was modified for specificity with Pholas orie...

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Bibliographic Details
Main Author: Girasol, Mark John G.
Format: Thesis
Language:English
Published: 2015.
Subjects:
Pholas Orientalis.
Restriction Fragment Lenght Polymorphisms (RFLPs)
Internal Transcribed Spacers (ITS)