RNA turnover in eukaryotes analysis of specialized and quality control RNA decay pathways - Maquat, Lynne; Kiledjian, Megerditch (San Diego, CA Academic Press, 2008)
Section I. : Analysis of specialized mRNA decay pathways 1. Methods to study no-go mRNA decay in Saccharomyces cervisiae / Meenakshi K. Doma -- 2. Cell-cycle regulation of histone mRNA degradation in mammalian cells: role of translation and oligouridylation / Thomas E. Mullen, Handan Kaygun, William F. Marzluff -- 3. Assays of adenylate uridylate-rich element-mediated mRNA decay in cells / Riza M. Ysla, Gerald M. Wilson, Gary Brewer -- 4. Evaluating the control of mRNA decay in fission yeast / Brandon J. Cuthbertson, Perry J. Blackshear -- 5. In Vivo analysis of the decay of transcripts generated by cytoplasmic RNA viruses / Nicole L. Carneau, Carol J. Wilusz, Jeffrey Wilusz -- Section II. : Nonsense-mediated mRNA decay (How do you study NMD; what defines an NMD target) -- 6. Qualitative and quantitative assessment of the activity of the yeast nonsense-mediated mRNA decay pathway / Feng He ... -- 7. Nonsense-mediated mRNA decay in Caenorhabditis elegans / Dasa Longman ... -- 8. In Vivo analysis of plant nonsense-mediated mRNA decay / Koichi Hori, Yuichiro Watanabe -- 9. Studying nonsense-mediated mRNA decay in mammalian cells / Daiki Matsuda, Hanae Sato, Lynne E. Maquat -- Section III. : Analysis of nuclear mRNA decay and non-mRNA Decay -- 10. Estimating nuclear mRAN decay in Saccharomyces cerevisiae / Manfred Schmidt ... [et al.] -- 11. Identification and analysis of tRNAs that are degraded in Saccharomyces cervisiae due to lach of modifications / Irina Chernyakov ... -- 12. Analysis of nonfunctional ribosomal RNA decay in Saccharomyces cerevisiae / Sarah E. Cole, Frederick J. LaRiviere -- Section IV. : Identifying targets of an RNA decay factor -- 13. Identifying substrates of mRNA decay factors by a combined RNA interference and DNA microarray approach / Jèurgen Whittmann, Hans-Martin Jèack -- 14. Analysis of RNA-protein interactions using a yeast three-hybrid system / Craig R. Stumpf, Laura Opperman, Marvin Wickens -- 15. Co-immunoprecipitation techniques for assessing RNA-protein interactions In Vivo / Nicholas K. Conrad -- Section V. : RNAi-mediated mRNA decay -- 16. How to define targets for small guide RNAs in RNA silencing: a biochemical approach / Mikiko C. Siomi, Kazumichi M. Nishida, Haruhiko Siomo -- 17. Extension of endogenous primers as a tool to detect micro-RNA targets / Sergei Vatolin, Robert J. Weil -- 18. Examining the influence of microRNAs on translation efficiency and on mRNA deadenylation and decay / Ligang Wu, Joel G.Belasco
Includes bibliographical references and indexes.
Keywords: Protista; RNA; Electronic books