RNA turnover in eukaryotes : nucleases, pathways and analysis of mRNA decay - Maquat, Lynne; Arraiano, Cecilia M. (San Diego, CA Elsevier/Academic Press, 2008)
Section I. Decapping analyses. Analysis of mRNA decapping / Shin-Wu Liu ... -- Kinetic assay to monitor DNA decapping under single-turnover conditions / Brittnee N. Jones ... -- Purification and analysis of the decapping activator Lsm1p-7p-Pat1p complex from yeast / Sundaresan Tharun -- Reconstitution of recombinant human LSm complexes for biochemical, biophysical, and cell biological studies / Bozidarka L. Zaric, Christian Kambach -- Section II. Polyadenylation/deadenylation analyses. Regulated deadenylation In Vitro / Aaron C. Goldstrohm, Brad A. Hook, Marvin Wickens -- Cell-free deadenylation assays with Drosophila embryo extracts / Mandy Jeske, Elmar Wahle -- Measuring CPEB-mediated cytoplasmic polyadenylation-deadenylation in Xenopus laevis oocytes and egg extracts / Jong Heon Kim, Joel D. Richter -- Preparation and applications of cytoplasmic extracts from mammalian cells for studying aspects of mRNA decay / Kevin J. Sokoloski, Jeffrey Wilusz, Carol J. Wilusz -- Section III. Nucleases in mRNA decay. In Vitro assays of 5' to 3' exoribonuclease activity / Olivier Pellegrini ... -- Reconstitution of RNA exosomes from human and Saccharomyces cervisiae: cloning, expression, purification, and activity assays / Jaclyn C. Greimann, Christophr D. Lima -- Biochemical studies of the mammalian exosome with intact cells /Geurt Schilders, Ger J.M. Pruijn -- Determining In Vivo activity of the yeast cytoplasmic exosome / Daneen Schaeffer ... -- Approaches for studying PMR1 endonuclease-mediated mRNA decay / Yuichi Otsuka, Daniel R. Schoenberg --Section IV. Measuring mRNA half-life In Vivo. Methods to determine mRNA half-life in Saccharomyces cerevisiaea / Jeff Coller -- mRNA decay analysis in Drosophila melanogaster: drug-induced changes in glutathione S-transferase D21 mRNA stability -- Bünyamin Akgül, chen-Pei D. Tu -- Measuring mRNA stability during early Drosophila embryogenesis / Jennifer L. Semotok ... -- Messenger RNA half-life measurements in mammalian cells / Chyi-Ying A. Chen, Nader Ezzeddine, Ann-Bin Shyu -- Trypanosomes as a model to investigate mRNA decay pathways / Stuart Archer ... -- Cell type-specific analysis of mRNA synthesis and decay In Vivo with uracil phosphoribosyltransferase and 4-thiouracil / Michael D.Cleary -- Section V. Defining degradative activities. Analysis of Cytoplasmic mRNA decay in Saccharomyces cerevisiae / Dario O Passos, Roy Parker -- Transcriptome targets of the exosome complex in plants / Dmitry Belostotsky -- Sensitive detectin of mRNA decay products by use of reverse-ligatin-mediated PCR (RL-PCR) / Thierry Grange -- Tethering assays to investigate nonsense-mediated mRNA decay activation proteins / Niels H. Gehring, Matthias W. Hentze, Andreas E. Kulozik -- Assays for determining Poly(A) tail length and the polarity of mRNA decay in mammalian cells / Elizabeth L. Murray, Daniel R. Schoenberg -- Section VI. Cell biology of RNA decay (i.e. translational repression/P bodies). Analyzing P-bodies in Saccaromyces cerevisiae / Tracy Nissan, Roy Parker -- Real-time and quantitative imaging of mammalian stress granules and processing bodies / Nancy Kedersha ... -- Cell biology of mRNA decay / David Grünwald, Robert H.Singer, Kevin Czaplinski
Includes bibliographical references and indexes.
Keywords: Messenger RNA; Genetic regulation; Eukaryotic cells; Nucleases; Electronic books