00000nmm a2200000 i 4500 UP-99804511805357566 Buklod 20230308151415.0 m o u cr || |||a|a|a 230308t20032003cau go u eng d 9780121827816 (part C) (OCoLC)54359635 UtOrBLW DSCI rda eng DMLUC QH 599 C47 2003eb Chromatin and chromatin remodeling enzymes. Part C edited by C. David Allis, Carl Wu. San Diego Elsevier/ Academic Press [2003] xxxviii, 546 pages, 7 unnumbered pages of plates illustrations (some color) text rdacontent computer rdamedia online resource rdacarrier Methods in Enzymology volume 377 Includes bibliographic references and index. 1. Functional analyses of chromatin modifications in yeast -- 2. Genetic analysis of chromatin remodeling using budding yeast as a model -- 3. Introduction to Trx-G and Pc-G genes -- 4. Genetic and cytological analysis of drosophila chromatin- remodeling factors -- 5. Genetic analysis if H1 linker histone subtypes and their functions in mice -- 6. The use of mass spectrometry for the analysis of histone modifications -- 7. Histone modification patterns during gene activation -- 8. Identification and analysis of native HAT complexes -- 9. Histone deacetylases: purification of the enzymes, substrates, and assay conditions -- 10. Measurement of mammalian histone deacetylase activity -- 11. Analysis of histone phosphorylation: coupling intracellular signaling to chromatin remodeling -- 12. Purification of histone methyltransferases from HeLa cells -- 13. Global proteomic analysis of s. cerevisiae (GPS) to identify proteins required for histone modifications -- 14. Purification of Sir2 proteins from yeast -- 15. Purification and biochemical properties of the drosophila TAC1 complex -- 16. Isolation and characterization of CHRASCH, a polycomb-containing silencing complex -- 17. Biochemical analysis of mammalian polycomb group protein complexes and the identification of genetic elements that block polycomb-mediated gene repression -- 18. Purification and functional analysis of the mammalian SWI/SNF-family of chromatin-remodeling complexes -- 19. Isolation and assay of the RSC chromatin-remodeling complex from saccharomyces cerevisiae -- 20. DNA translocation and nucleosome remodeling assays by the RSC chromatin remodeling complex -- 21. A Necleosome sliding assay for chromatin remodeling factors -- 22. Methods for analysis of nucleosome sliding by drosophila NURF -- 23. Methods for preparation and assays for xenopus ISWI complexes -- 24. Functional analysis or ISWI complexes in mammalian cells -- 25. Preparation and assays for mammalian ISWI complexes -- 26. Preparation and analysis of the INO80 complex -- 27. Assay of Z-DNA induction by chromatin remodeling factors -- 28. Immuno-depletion and purification strategies to study chromatin-remodeling factors in vitro -- 29. Transcription through the nucleosome by mRNA-producing RNA polymerases -- 30. Reconstitution and transcriptional analysis of chromatin in vitro -- 31. Techniques used to study transcription on chromatin templates -- 32. Analysis of DNA repair on nucleosome templates. IP-based subscription, on campus only. Access via Electronic Resources of the UPD University Library Website. Chromatin. Histones. Electronic books. Allis, C. David. editor. Wu, Carl. editor. Methods in enzymology volume 377. https://www.sciencedirect.com/bookseries/methods-in-enzymology/vol/377/suppl/C Available remotely for University of the Philippines System via Science Direct. Click here to access through Open Athens (viewed 15 June 2022) FO UPD DSCI QH 599 C47 2003EB Electronic Resource