Chromatin and chromatin remodeling enzymes. Part C - Allis, C. David; Wu, Carl. (San Diego Elsevier/ Academic Press, 2003)
1. Functional analyses of chromatin modifications in yeast -- 2. Genetic analysis of chromatin remodeling using budding yeast as a model -- 3. Introduction to Trx-G and Pc-G genes -- 4. Genetic and cytological analysis of drosophila chromatin- remodeling factors -- 5. Genetic analysis if H1 linker histone subtypes and their functions in mice -- 6. The use of mass spectrometry for the analysis of histone modifications -- 7. Histone modification patterns during gene activation -- 8. Identification and analysis of native HAT complexes -- 9. Histone deacetylases: purification of the enzymes, substrates, and assay conditions -- 10. Measurement of mammalian histone deacetylase activity -- 11. Analysis of histone phosphorylation: coupling intracellular signaling to chromatin remodeling -- 12. Purification of histone methyltransferases from HeLa cells -- 13. Global proteomic analysis of s. cerevisiae (GPS) to identify proteins required for histone modifications -- 14. Purification of Sir2 proteins from yeast -- 15. Purification and biochemical properties of the drosophila TAC1 complex -- 16. Isolation and characterization of CHRASCH, a polycomb-containing silencing complex -- 17. Biochemical analysis of mammalian polycomb group protein complexes and the identification of genetic elements that block polycomb-mediated gene repression -- 18. Purification and functional analysis of the mammalian SWI/SNF-family of chromatin-remodeling complexes -- 19. Isolation and assay of the RSC chromatin-remodeling complex from saccharomyces cerevisiae -- 20. DNA translocation and nucleosome remodeling assays by the RSC chromatin remodeling complex -- 21. A Necleosome sliding assay for chromatin remodeling factors -- 22. Methods for analysis of nucleosome sliding by drosophila NURF -- 23. Methods for preparation and assays for xenopus ISWI complexes -- 24. Functional analysis or ISWI complexes in mammalian cells -- 25. Preparation and assays for mammalian ISWI complexes -- 26. Preparation and analysis of the INO80 complex -- 27. Assay of Z-DNA induction by chromatin remodeling factors -- 28. Immuno-depletion and purification strategies to study chromatin-remodeling factors in vitro -- 29. Transcription through the nucleosome by mRNA-producing RNA polymerases -- 30. Reconstitution and transcriptional analysis of chromatin in vitro -- 31. Techniques used to study transcription on chromatin templates -- 32. Analysis of DNA repair on nucleosome templates.
Includes bibliographic references and index.
Keywords: Chromatin; Histones; Electronic books