00000nmm a2200000 i 4500 UP-99804511805352578 Buklod 20230308145602.0 m o u cr || |||a|a|a 230308t20032003cau go u eng d 9780121827809 (part B) (OCoLC)54489790 UtOrBLW DSCI rda eng DMLUC QH 599 C47 2003eb Chromatin and chromatin remodeling enzymes part B edited by C. David Allis, Carl Wu. San Diego, CA Elsevier/ Academic Press [2003] 1 online resource (xvi, 458 pages, 27 unnumbered pages of plates) illustrations (some color) text txt rdacontent computer rdamedia online resource rdacarrier Methods in enzymology volume 376 Includes bibliographic references and index. 1. Fluorescence anisotropy assays for analysis of ISWI-DNA and ISWI-nucleosome interactions -- 2. Biophysical analysis of specific genomic loci assembled as chromatin in vivo -- 3. Visualization and 3D structure determination of defined sequence chromatin and chromatin remodeling complexes -- 4. Electron microscopic analysis of the RSC chromatin remodeling complex -- 5. Use of optical trapping techniques to study single-nucleosome dynamics -- 6. Single-molecule analysis of chromatin -- 7. Biochemical and structural characterization of recombinant histone acetyltransferase proteins -- 8. Use of nuclear magnetic resonance spectroscopy to study structure-function of bromodomains -- 9. Assays for the determination of structure and dynamics of the interaction of the chromodomain with histone peptides -- 10. Expression, purification, and biophysical studies of chromodomain proteins -- 11. Quantitative assays for characterization of the Sir2 family of NAD+-dependent deacetylases -- 12. Selective HAT inhibitors as mechanistic tools for protein acetylation -- 13. Histone deacetylase inhibitors: assays to assess effectiveness in vitro and in vivo -- 14. Immunochemical analysis of chromatin -- 15. Generation and characterization of antibodies directed against di-modified histones, and comments on antibody and epitope recognition -- 16. Generation and characterization of methyllysine histone antibodies -- 17. Tips in analyzing antibodies directed against specific histone tail modifications -- 18. Histone methylation: recognizing the methyl mark -- 19. Analysis of genome-wide histone acetylation state and enzyme binding using DNA microarrays -- 20. Use of chromatin immunoprecipitation assays in genome- wide location analysis of mammalian transcription factors -- 21. High-throughput screening of chromatin immunoprecipitates using of CpG-island microarrays -- 22. Chromatin immunoprecipitation in the analysis of large chromatin murine antigen receptor loci -- 23. The use of chromatin immunoprecipitation assays in genome-wide analyses of histone modifications -- 24. Sequential chromatin immunoprecipitation from animal tissues -- 25. Immunofluorescent staining of polytene chromosomes: exploiting genetic tools -- 26. Indirect immunofluorescent labeling of drosophila polytene chromosomes: visualizing protein interactions with chromatin in vivo -- 27. X-chromosome inactivation in mouse embryonic stem cells: analysis of histone modifications and transcriptional activity using immunofluorescence and FISH -- 28. X inactivation in mouse ES cells: histone modifications and FISH. IP-based subscription, on campus only. Access via Electronic Resources of the UPD University Library Website. Chromatin. Enzymology. Histones. Electronic books. Allis, C. David. editor. Wu, Carl. editor. Methods in enzymology volume 376. https://www.sciencedirect.com/bookseries/methods-in-enzymology/vol/376/suppl/C Available remotely for University of the Philippines System via Science Direct. Click here to access through Open Athens (viewed 08 June 2022) FO UPD DSCI QH 599 C47 2003EB Electronic Resource