Chromatin and chromatin remodeling enzymes part B - Allis, C. David; Wu, Carl. (San Diego, CA Elsevier/ Academic Press, 2003)
1. Fluorescence anisotropy assays for analysis of ISWI-DNA and ISWI-nucleosome interactions -- 2. Biophysical analysis of specific genomic loci assembled as chromatin in vivo -- 3. Visualization and 3D structure determination of defined sequence chromatin and chromatin remodeling complexes -- 4. Electron microscopic analysis of the RSC chromatin remodeling complex -- 5. Use of optical trapping techniques to study single-nucleosome dynamics -- 6. Single-molecule analysis of chromatin -- 7. Biochemical and structural characterization of recombinant histone acetyltransferase proteins -- 8. Use of nuclear magnetic resonance spectroscopy to study structure-function of bromodomains -- 9. Assays for the determination of structure and dynamics of the interaction of the chromodomain with histone peptides -- 10. Expression, purification, and biophysical studies of chromodomain proteins -- 11. Quantitative assays for characterization of the Sir2 family of NAD+-dependent deacetylases -- 12. Selective HAT inhibitors as mechanistic tools for protein acetylation -- 13. Histone deacetylase inhibitors: assays to assess effectiveness in vitro and in vivo -- 14. Immunochemical analysis of chromatin -- 15. Generation and characterization of antibodies directed against di-modified histones, and comments on antibody and epitope recognition -- 16. Generation and characterization of methyllysine histone antibodies -- 17. Tips in analyzing antibodies directed against specific histone tail modifications -- 18. Histone methylation: recognizing the methyl mark -- 19. Analysis of genome-wide histone acetylation state and enzyme binding using DNA microarrays -- 20. Use of chromatin immunoprecipitation assays in genome- wide location analysis of mammalian transcription factors -- 21. High-throughput screening of chromatin immunoprecipitates using of CpG-island microarrays -- 22. Chromatin immunoprecipitation in the analysis of large chromatin murine antigen receptor loci -- 23. The use of chromatin immunoprecipitation assays in genome-wide analyses of histone modifications -- 24. Sequential chromatin immunoprecipitation from animal tissues -- 25. Immunofluorescent staining of polytene chromosomes: exploiting genetic tools -- 26. Indirect immunofluorescent labeling of drosophila polytene chromosomes: visualizing protein interactions with chromatin in vivo -- 27. X-chromosome inactivation in mouse embryonic stem cells: analysis of histone modifications and transcriptional activity using immunofluorescence and FISH -- 28. X inactivation in mouse ES cells: histone modifications and FISH.
Includes bibliographic references and index.
Keywords: Chromatin; Enzymology; Histones; Electronic books