00000cmm a22000004i 4500 UP-99796217613855380 Buklod 20231109081254.0 m o u cr ||||||||||| 231109s2015 mau u a eng d 9780128024508 (iLib)UPD-00528671895 UtOrBLW DSCI rda eng DMLUC QH 585.2 B85 2015eb Building a cell from its component parts edited by Jennifer Ross and Wallace F. Marshall. Waltham, MA Academic Press is an imprint of Elsevier [2015] 1 online resource (xviii, 386 pages, 23 pages of plates) illustrations text rdacontent computer rdamedia online resource rdacarrier Methods in cell biology volume 128 Includes bibliographical references and index. In vitro systems for the study of microtubule-based cell polarity in fission yeast / Nuria Taberner, Andies Lof, Sophie Roth, Dimitry Lamers, Hans Zeijlemaker, Marleen Dogterom -- Microtubules, MAPs, and motor patterns / Kasimira T. Stanhope, Jennifer L. Ross -- Self-organization of motors and microtubules in lipid-monolayered droplets / Hella Baumann, Thomas Surrey -- Reconstitution of microtubule-based motility using cell extracts / Swathi Ayloo, Erika L.F. Holzbaur -- Building cells for quantitative, live-cell analyses of collective motor protein functions / Eric A. Kumar, David Tsao, Anand Radhakrishnan, Michael Diehl -- Reconstructing cytoskeletal contraction events with biomimetic actin - myosin active gels / Jose Alvarado, Gijsje H. Koenderink -- Building an artificial actin cortex on microscopic pillar arrays / R. Ayadi, W.H. Roos -- Triggering actin polymerization in xenopus egg extracts from phosphoinositide-containing lipid bilayers / Astrid Walrant, Daniel S. Saxton, Guilherme Pereira Correia, Jennifer L. Gallop -- Reconstructing geometry-modulated protein patterns in membrane compartments / Katja Zieske, Petra Schwille -- Structural and functional studies of membrane remodeling machines / Raghav Kalis, Nathaniel Talledge, Adam Frost -- Building interconnected membrane networks / Matthew A. Holden -- Using supported bilayers to study the spatiotemporal organization of membrane-bound proteins / Phuong A. Nguyen, Christine M. Field, Aaron C. Groen, Timothy J. Mitchison, Martin Loose -- Reconstituting parA/parB-mediated transport of DNA cargo / Anthony G. Vecchiarelli, James A. Taylor, Kiyoshi Mizuuchi -- Cell-sized liposomes that mimic cell motility and the cell cortex / Joel Lemiere, Kevin Carvalho, Cecile Sykes -- Reconstitution of cortical actin networks within water-in-oil emulsions / Enas Abu Shah, Maya Malik-Garbi, Kinneret Keren -- Engineering artificial cells by combining HeLa-based cell-free expression and ultrathin double emulsion template / Kenneth K.Y. Ho, Victoria L. Murray, Allen P. Liu -- Reconstitution of proteins on electroformed giant unilamellar vesicles / Eva M. Schmid, David L. Richmond, Daniel A. Fletcher -- Reconstituting SNARE-mediated membrane fusion at the single liposome level / Vilker Keissling, Binyong Liang, Lukas K. Tamm IP-based subscription, on campus only. Access via Electronic Resources of the UPD University Library Website. The cell interior is another world that we are only beginning to explore. Although there are a number of approaches for examining the inner workings of the cell, the reductionist approach of building up complexity appeals to many with physical science and engineering backgrounds. This volume of Methods in Cell Biology spans a range of spatial scales from single protein molecules to vesicle and cell sized structures capable of complex behaviors. Contributions include; methods for combining different motors and cytoskeletal components in defined ways to produce more complex behaviors; methods to combine cytoskeletal assemblies with fabricated devices such as chambers or pillar arrays; reconstituting membrane fission and fusion; reconstituting important biological processes that normally take place on membrane surfaces; and methods for encapsulating protein machines within vesicles or droplets.--Provided by publisher. Cell culture. Electronic books. Ross, Jennifer editor. Marshall, Wallace F. editor. Methods in cell biology volume 128. Available for University of the Philippines System via Science Direct. Also available remotely thru Open Athens: https://www.sciencedirect.com/bookseries/methods-in-cell-biology/vol/128/suppl/C FO UPD DSCI Electronic Resource