Mitochondria - Pon, Liza A.; Schon, Eric A. (San Diego, CA Academic Press, 2001)
1. Isolation and subfractionation of mitochondria from animal cells and tissue culture lines -- 2. Isolation and subfractionation of mitochondria from the yeast Saccharomyces cerevisiae -- 3. Isolation and subfractionation of mitochondria from plants -- 4. Assessing functional integrity of mitochondria in vitro and in vivo -- 5. Assaying mitochondrial respiratory complex activity in mitochondria isolated from human cells and tissues -- 6. In vivo measurements of respiration control by cytochrome c-oxidase and in situ analysis of oxidative phosphorylation -- 7. Assay of mitochondrial ATP synthesis in animal cells -- 8. Measurement of membrane permeability and permeability transition of mitochondria -- 9. Assaying actin-binding activity of mitochondria in yeast -- 10. Analysis and prediction of mitochondrial targeting peptides -- 11. Assaying protein import into mitochondria -- 12. Analysis of protein-protein interactions in mitochondria by coimmunoprecipitation and chemical cross-linking -- 13. Blue-native gels to isolate protein complexes from mitochondria -- 14. Application of electron tomography to mitochondrial research -- 15. Epitope tagging and visualization of nuclear-encoded mitochondrial proteins in yeast -- 16. Targeting of green fluorescent protein to mitochondria -- 17. Assessment of mitochondrial membrane potential in situ using single potentiometric dyes and a novel fluorescence resonance energy transfer technique -- 18. Optical imaging techniques (histochemical, immunohistochemical, and in situ hybridization staining methods) to visualize mitochondria -- 19. Visualization of mitochondrial movement in yeast -- 20. Targeting of reporter molecules to mitochondria to measure calcium, ATP, and pH -- 21. Genetic transformation of Saccharomyces cerevisiae mitochondria -- 22. Transmitochondrial technology in animal cells -- 23. Diagnostic assays for defects in mitochondrial DNA replication and transcription in yeast and human cells -- 24. Analysis of mitochondrial translation products in vivo and in organello in yeast -- 25. Numerical methods for handling uncertainty in microarray data: an example analyzing perturbed mitochondrial function in yeast.
"Prepared under the auspices of the American Society for Cell Biology."
Includes bibliographical references and index.
Keywords: Mitochondria; Cytology -- Technique; Electronic books