Live cell imaging a laboratory manual - Goldman, Robert D.; Swedlow, Jason; Spector, David L. (Cold Spring Harbor, N.Y. Cold Spring Harbor Laboratory Press, 2010)
Fluorescent protein tracking and detection -- Constructing and expressing fluorescent protein fusions -- Micropatterning cell-substrate adhesions using linear polyacrylamide as the blocking agent -- CCD cameras for fluorescent imaging of living cells -- Fluorescence perturbation techniques to study mobility and molecular dynamics of proteins in live cells: FRAP, photoactivation, photoconversion, and FLIP -- Imaging protein states in cells -- A versatile, multicolor total internal reflection fluorescence and spinning-disk confocal microscope system for high-resolution live cell imaging -- Confocal microscopy, deconvolution, and structured illumination methods -- Atomic-force microscopy for biological imaging and mechanical testing across length scales -- OMX: a new platform for multimodal, multichannel wide-field imaging -- Digital scanned laser light sheet fluorescence microscopy -- First steps for fluorescence correlation spectrocsopy of living cells -- Tracking and quantitative analysis of dynamic movements of cells and particles -- Imaging techniques for measuring the materials properites of cells -- Computational image analysis of cellular dynamics: a case study based on particle tracking -- Software tools, data structures, and interfaces for microscope imaging -- High-throughput microscopy using live mammalian cells -- In vivo imaging of mammalian cells -- Live cell imaging of yeast -- Live imaging of Caenorhabditis elegans -- Live cell imaging of plants -- Pushing the limits of live cell imaging in Drosophila -- Dynamic, long-term, in vivo imaging of tumor-stroma interactions in mouse models of breast cancer using spinning-disk confocal microscopy -- High-resolution multiphoton imaging of tumors in vivo -- Correlated live cell light and electron microscopy using tetracysteine tags and biarsenicals -- Intravital microscopy of normal and diseased tissues in the mouse -- Imaging lipids in living cells -- Development of mammalian cell lines with Iac operator-tagged chromosomes -- Imaging gene expression in living cells --Studying mitosis in cultured mammalian cells -- Imaging intermediate filament proteins in living cells -- Methods for expressing and analyzing GFP-tubulin and GFP-microtubule-associated proteins -- Imaging of membrane systems and membrane traffic in living cells -- Imaging live cells under mechanical stress -- Imaging single molecules using total internal reflection fluorescence microscopy -- Cellular imaging using total internal reflection fluorescence microscopy -- Visualization and quantification of single RNA molecules in living cells.
Includes bibliographical references and index.
Keywords: Cells -- Laboratory manuals; Microscopy -- Laboratory manuals; Cells -- Laboratory manuals; Microscopy -- Methods -- Laboratory manuals