00000ctmaa22000004a 4500 UP-99796217610396498 Buklod 20120712092451.0 m |o d | ta 120712s2006 xx d r |||| u| (iLib)UPD-00187104728 DML eng DMLUC LG 995 2006 C4 E87 Espino, Samuel S. Novel toxin genes, biological activity of putative toxins and molecular phylogeny of Conus furvus Reeve, 1843 by Samuel S. Espino. 2006. xiii, 144 leaves ill. "October 2006." Thesis (M.S. Chemistry)--University of the Philippines Diliman. The genus Conus comprises a remarkable group of predatory marine gastropods characterized by a special biochemistry of small constrained peptides, the conotoxins. These conotoxins have an extremely high affinity to a particular ion channel in the snail's prey. In this research, conotoxins from Conus furvus were deduced from cDNA sequences. Conotoxins of the O- and the M - superfamily were obtained. The O-superfamily conotoxin has the sequence SCTPRGGQCGYYNDCCSHQCNINRNLCE and the M - superfamily conotoxin has the sequence RCCKFPCANSCRHLCCG. The M - superfamily conotoxin has a high degree of identity and similarity with conotoxins expressed by the mollusc hunting snails Conus textile and Conus marmoreus. HPLC fractions obtained from the snail's venom show activities ranging from sluggish and sleep - like behavior to hyperactivity and convulsion in mice. Phylogenetic analyses utilizing several mithochondrial DNA sequences indicate that C. furvus preys on molluscs. Conus Phylogeny Molecular aspects. Conus Nomenclature. Marine toxins Analysis. Snails. FI UP Thesis UPD DARCHIVES LG 995 2006 C4 E87 Thesis