00000ctmaa22000004a 4500 UP-99796217610396020 Buklod 20120706104811.0 m |o d | ta 120706s2007 xx d r |||| u| (iLib)UPD-00187104193 DML eng DMLUC LG 995 2007 M53 R44 Regalia, Raymond Sabio 1975- Characterization of marine bacterial isolate, Flammeovirga sp. UPMSI-L001 and assay of its carrageenolytic activity by Raymond Sabio Regalia. 2007. "June 2007." Vita. Thesis (M.S. in Microbiology)--University of the Philippines Diliman. A carrageenan-degrading marine bacterium, UPMSI-L001, previously isolated from ice-ice-infected thalli of Kappaphycus alvarezii was characterized. This bacterium which forms colonies with deep and wide depression on solidified culture medium is Gram-negative, rod-shaped, aerobic, chemoorganotropic and non-flagellated but apparently motile by gliding. It is capable of growth at 25ºC and 30ºC and at6 NaCl concentrations between 1-10%. Phylogenetic analysis of its 16S rDNA sequence revealed its close affiliation with the genus Flammeovirga. It is however distinct from its closest relative, Flammeovirga yaeyamensis by being non-pigmented and urease-positive and by having an oxidative type of glucoes utilization. The carrageenase production in liquid cultures. Several culture media were compared and the highest carrageenase activity (127.6 U/ml) was obtained in a seawater-based culture medium (SWM) supplemented with yeast extarct (5g/L) and trytone (5g/L). It was also found that this isolate had iota-carrageenase (59.7/ml) and lambda-carrageenase(54.7/ml) activities. This study is the first report on a Flammeovirga species that is associated with ice-ice disease in Kappaphycus alvarezii and has broad-range carrageenolytic activity. This strain has the potential to be used for the production of carrageenases for various biotechnological applications. FI UP Thesis UPD DARCHIVES LG 995 2007 M53 R44 Thesis