00000nam a22000004a 4500 UP-8027390931316422134 Buklod 20251001112657.0 m |o d | ta 250924s2025 xxu r |||| u|eng d PHARM rda eng LG 993.5 2025 P5 A95 Aujero, Maude Angela author Evaluation of the anticancer potentian of antioxidant-rich fractions in the methanolic leaf extract of Piper betle L. (Piperraceae) Maude Angela Aujero, Sheanne Kyra P. Cabantac ; Paolo Robert P. Bueno, adviser. Manila Department of Pharmacy, College of Pharmacy, University of the Philippines Manila 2025 xvi, 119 leaves 30 cm. Thesis (Bachelor of Science in Pharmaceutical Science)--University of the Philippines Manila, July 2025 available to general public In the Philippines, cancer is the second highest cause of mortality, and is the leading type of cancer among Filipino women. Despite the high burden, cancer treatment remains challenging as treatment plans vary and side effects pose harm on top of the disease itself. With this, Piper betle (P. betle) was studied through an assay-guided evaluation for its antioxidant activity to ascertain its potential as an anticancer agent. The methanolic leaf extract of P. betle was obtained and subjected to antioxidant assays (FRAP assay and DPPH assay) to determine the solvent fraction with the highest antioxidant activity. The solvent fraction with the highest antioxidant activity was separated using open column chromatography. The pooled fractions (sub-fractions) were further subjected to antioxidant assays and Nitric Oxide radical scavenging assay. The fractions with the highest antioxidant activity was evaluated for its cytotoxicity using the MTT assay. Phytochemical testing revealed the crude extract to contain phenolics, alkaloids, and flavonoids. The ethyl acetate solvent fraction of P. betle provided the highest antioxidant activity in both FRAP assay and the DPPH assay (IC50 = 12.30 μg/mL). Assay for total phenolics via the Folin-Ciocalteu method also revealed the ethyl acetate layer to have the highest total phenolic content at 430.1014 ± 45.36 mg Gallic Acid Equivalent/g (GAE/g). Ethyl acetate was then subjected to semi-purification using open column chromatography. A total of 13 pooled fractions was obtained. Sub-fractions 15-23 (SF 15-23) presented the highest antioxidant activity in the FRAP assay with the highest FeSO4 equivalent of 9.0117 mM FeSO4 /g. It also presented the highest scavenging capacity at 70.8649 ± 1.37% in the DPPH scavenging assay. The Nitric Oxide radical scavenging assay further assessed the ability of SF15-23 to inhibit the production of nitric oxide radicals which yielded a scavenging capacity of 30.7149 ± 2.14%. Based on these results, SF15-23 were chosen for cytotoxicity testing using the MTT assay. MCF-7 breast cancer cell lines treated with SF15-23 showed an inhibition of 94.5114 ± 4.0583% with an IC50 computed at 64.48 μg/mL. Provided with these findings, P. betle was able to demonstrate an anti-cancer potential. Further studies can be done by isolating and characterizing the compounds found in the sub-fractions exhibiting anti-cancer potential. Other cancer cell lines can also be used to explore the cytotoxicity of the active sub-fractions which can be applied in other types of cancer. Piper betle Methanolic extracts Antioxidants Anticancer activity. Antineoplastic agents Plant-derived Phytochemistry Philippines. Medicinal plants Pharmacological evaluation Antioxidants Philippines. Cabantac Sheanne Kyra P. author . Bueno, Paolo Robert P. adviser. FI UP UPMNL PHARM LG 993.5 2025 P5 A95 Thesis