00000nam a22000004a 4500 UP-8027390931316420881 Buklod 20250930100049.0 m |o d | cr ||||||||||| 250924s2023 ph o erm |||| u|eng d UP CPH rda eng LG 993.5 2023 P85 D45 Dela Cruz, Angela Lorraine G. author. Antimicrobial resistance profile of Escherichia coli isolated from raw chicken meat in a selected wet market in Manila City, Philippines Angela Lorraine G. Dela Cruz, Lynder Kyle A. Dimaapi, Roger Andrei D. Francisco, Rei Gilian D. Noble, Hayley Emerald G. Sabangan; Maria Margarita M. Lota, MD, FPPD, MHPED, Azita Racquel Gavino-Lacuna, RMT, DMM, MPH, advisers. 2023 Manila College of Public Health, University of the Philippines Manila xvi, 288 leaves colored illustrations 28 cm. text rdacontent unmediated rdamedia volume rdacarrier Includes references and appendices. Special Study Bachelor of Science in Public Health College of Public Health, University of the Philippines Manila 2023 Background: Antimicrobial resistance (AMR) is a leading global public health concern as it resulted in more difficult-to-treat infections and fatalities. In the Philippines, drug-resistant E. coli, including multidrug resistant (MDR), extended-spectrum beta-lactamase (ESBL)-producing, carbapenemase-producing carbapenem-resistant (CP-CR) E. coli, have been isolated from common food animals, increasing the risk of cross-contamination between humans, animals, and the environment. However, there is a lack of data on the distribution of E. coli in chicken meat in public wet markets. This study aims to describe the AMR profile of E. coli in raw chicken meat from retail stalls in a selected wet market in Manila City. Methods: This quantitative descriptive study characterized the AMR profile of E. coli isolated from 25 raw chicken meat samples from a wet market in Manila City. Antimicrobial susceptibility was determined through disk diffusion method against 23 antimicrobial agents in 16 antimicrobial classes. MDR E. coli were identified based on the resistance patterns. ESBL and carbapenemase-producing capacities of the bacteria were tested through double-disk synergy test and modified carbapenem inactivation method, respectively. Results: Twenty-four out of 25 (96%) chicken samples contained E. coli isolates. Of these, 23 (96%) were classified as MDR. High resistance rates were observed against ampicillin (92%), tetracycline (88%), trimethoprim-sulfamethoxazole (83%), chloramphenicol (79%), ampicillin-sulbactam (75%), amoxicillin-clavulanic acid (67%), fosfomycin (67%), and streptomycin (54%). The majority of the E. coli isolates were still susceptible to a wide range of selected antimicrobial agents, including carbapenems, most cephalosporins, piperacillin-tazobactam, aztreonam, cefoxitin, and nitrofurantoin, among others. Meanwhile, none of the 24 isolated E. coli samples were classified as ESBL- and CP-CR E. coli. Conclusion: The high rates of AMR E. coli isolated in this study suggest that chickens in wet markets can potentially serve as reservoir hosts for drug-resistance genes, which could transfer to other bacteria and contaminate humans, animals, and the environment within the food production and supply chain. These findings emphasize the need for AMR surveillance and strategies to combat AMR in the Philippines through the One Health approach. Keywords: Antimicrobial resistance, multi-drug resistance, ESBL, carbapenemase, E. coli Escherichia coli Drug resistance Philippines Manila. Chickens Contamination Philippines Manila. Food Contamination Microbiology Philippines Manila. Drug resistance in microorganisms Philippines Manila. Manila (Philippines) Markets Health and hygiene. Dimaapi, Lynder Kyle A. author. Francisco, Roger Andrei D. author. Noble, Rei Gilian D. author. Sabangan, Hayley Emerald G. author. Lota, Maria Margarita M. adviser. Gavino-Lacuna, Azita Racquel adviser. FI UP UPMNL CPH LG 993.5 2023 P85 D45 Thesis