00000ctm a22000004a 4500 UP-8027390931316151458 Buklod 20071106064409.0 a r |||| u| ta 071106s xx d r |||| u| (iLib)UPMIN-00000014743 DLC DLC upmin eng LG993.5 2007 B4 B66 Bongcawil, Roxanne Yen C. In vitro morphological studies and cultural characterization of Pestalotiopsis Sp. associated with Nepenthes truncata macf. Roxanne Yen C. Bongcawil. 2007 40 leaves Thesis (BS Biology) -- University of the Philippines Mindanao, 2007 A fungus pestalotiopsis sp. from brown leafspot-infected pitcher plants (Nephentes truncate macf.) in Marilog District, Davao City was isolated to pure culture. Fungus growth in plated Potato Dextrose Agar (PDA) appeared white and cottony with punctiform acervuli. Under microscopy, the fungus hypha is hyaline, septated with hyphal cells measuring 23.99 (1.40)m wide. The conidia, which define the genius, are fusiform with 3 yellowish-brown median cells and hyaline appendages at opposite ends. The basal portion having 1 and the apical portion with 2 to 3 appendages. Total spore size is 22.09 ((1.73)m long and 6.11 (0.64)m wide. The 3 median cells measured 15 (1.17)m long. The basal appendage is 5.04 (1.69)m long while the apical appendages ranged from 9.54 to 34.09m, with an average of 20.41 (6.35)m ling. In axenic PDA medium, cultures grown under alternating 12-hr light and 12-hr darkness had the widest colonies, however, spore production was highest with continuous light after a 7-day growth time. No spore was observed on cultures under continuous darkness. Colony growth was widest and spore production highest with cultures under pH 8. Mycelial growth was not significantly influenced by culture temperatures, 230C and 27oC, however, spore production was higher in the former Gungi. Nepenthes species. Fungal diseases. Pathogenicity. Inoculum. Inoculation. Reisolation. Vitro. Morphological Studies. Germination. Undergraduate Thesis BIO200 BSB. FI UP UPMIN UPMIN-MAIN LG993.5 2007 B4 B66 Thesis