00000ctm a22000003a 4500 UP-8027390931314002785 Buklod 20180129145640.0 m |o d | ta 180129s xx d r |||| u| (iLib)UPVIS-00052361752 Gift 6.27.17 VML/ess 01.29.18 eng LG 993 2017 B4 J67 Joson, Santiago Emil A IV Antimicrobial assay of endophytic fungi isolated from selected mangroves in Iloilo River. Santiago Emil A Joson, IV. 2017. viii, 29 leaves tables, pictures and comouter print-out. Special Problem (Undergraduate, Bachelor of Science in Biology) College of Arts and Sciences, University of the Philippines Visayas, Miagao, Iloilo Endophytic fungi, especially from host plants of high priority screening (such as medicinal plants and plants with unique adaptations to survive), are top targets for bioprospecting beneficial secondary metabolites. Endophytic fungi were isolated from four mangrove hosts along Iloilo River: Avicennia officinalis, Avidennia rumphiana, Heretiera litoralis, and Xylocarpus granatum. Ten dominant fungal isolates were chosen for preliminary screening of antimicrobial properties against bacteria (Escherichia coli, Pseudomonas auruginosa, Enterococcus faecium, Staphylococcus aureus) and one fungus (Candida albicans) Among the tested isolates, only one isolate (Aspergillus candidus) exhibited potential and proceeded for further testing. The fungus was grown in liquid medium (PD Broth) for one week and the filtrate was concentrated using ethyl acetate as a diluents. The disk diffusion assay was used to test the crude extract at varying concentrations (100%, 75%, 50%, 25%) and compared with positive and negative controls. This paper reports that the crude extract exhibited weak inhibitory activity against E. coli compared with the activity of the positive control. However, there is a need to isolate and test more fungal endophytes from other Philippines mangroves for their potential to produce more potent antimicrobial. Fungi. Endophyte. Mangrove. Antimicrobial. Sadaba, Resurreccion Adviser. UPVIS UPV-ML LG 993 2017 B4 J67 Thesis