00000ctm a22000003a 4500 UP-8027390931313988540 Buklod 20170809134546.0 m |o d | ta 170809s xx d r |||| u| (iLib)UPVIS-00048522226 Gift VML/ess 8.9.17 eng LG 993 2014 B4 G37 Gardoce, Rosthon Subron Cryopreservation viability of methicillin-resistant Staphylococcus aureus under varying concentrations of dimethyl sulfoxide. Rosthon Subron Gardoce. 2014. vii, 43 leaves computer print-out. Special Problem (Undergraduate, Bachelor of Science in Biology) College of Arts and Sciences, University of the Philippines Visayas, Miagao, Iloilo Staphylococcus aureus is a pathogen of increasing importance due to a rise in antibiotic resistance and multidrug resistant strains of Methcillin-Resistant Staphylococcus aureus (MRSA). In a hospital, medical and agricultural setting, MRSA infestation can lead to severe problems which could even result to death. The significant impact of MRSA on public health and epidemiology suggests that a long-term preservation method is needed for extensive studies on the said species. The knowledge on the optimum cryopreservaton conditions for MRSA will be useful for long-term preservation of cell cultures for future studies. Hence, the optimum cryopreservation condition for MRSA was investigated. MRSA isolates were treated with different dimethyl sulfoxide (DMSO) concentrations ( 0 percent, 5 percent, 10 percent, 15 percent, 20 percent) at three different levels of storage temperature (4 degrees centigrade, -20 degrees centigrade, -80 degrees centigrade) for a period of 150 days. Three-Way Analysis of variance revealed that the interaction between the said factors had a significant effect on the cell viability and density of MRSA. Turkey's Test revealed that the optimum condition for cryopreservation of MRSA that maintained the highest percentage of cell viability and population is at 5 percent DMSO at -20degrees centigrade. Moreover, cryopreservation up to 30 days is the optimum condition for MRSA to be viable. Certain factors such as pH are recommended to be included in the study to further test an effective cryopreservation protocol for MRSA. Staphylococcus Aureus. Methicillin-Resistant Staphylococcus Aureus. Cryopreservation. Padilla, Philip Ian P. Adviser. UPVIS UPV-ML LG 993 2014 B4 G37 Thesis