00000ctm a22000004a 4500 UP-8027390931312570104 Buklod 20240321094245.0 o--- | || || ta 240321s2023 xx d r |||| u| (iLib)UPCEB-00012909775 UPC eng LG 993 2023 B4 A87 Asparin, Jade Frances B.. author. Bio-retting of Philippine corn husk fibers (Zea mays L.) using Bacillus cereus and its effects on fiber properties Jade Frances B. Asparin; thesis adviser, Fleurdeliz F. Maglangit. 2023. Cebu City, Cebu Department of Biology and Environmental Science, College of Science, University of the Philippines Cebu c2023. viii, 51 leaves illustrations (some col.) Thesis (Bachelor of Science in Biology)--University of the Philippines Cebu. July 2023. Not available to the general public. Available only after consultation with author/ research adviser. Available only to those bound by confidentiality agreement. Synthetic fibers are commonly used for textile manufacturing. However, these fibers pose greater risks to humans and the environment compared to natural fibers. While natural fibers like cotton are a better alternative, increasing land scarcity makes it unfeasible to plant more to meet textile demand. Additionally, the extraction of these fibers also introduces harmful chemicals into the environment. Thus, this study aims to biologically extract fibers from Philippine corn husks of a Visayan sweet corn variety and compare it with the chemical method. Corn husks samples were collected from decayed corn husks in Liloan, Cebu. The isolated stains were screened for laccase and pectinase activities using Toluidine Blue O Dye and Yeast Extract Pectin agar media, respectively. For the biological retting, 5 g of corn husk fibers were submerged in a Bushnell-Haas Broth culture for 2 days at 37˚C, while another 5 g were treated with 5% NaOH solution at room temperature (1 h, MLR: 1:40) for the chemical rating. The effect of B. cereus on the weight loss of cornhusk-treated fibers was found to be 21.51 ± 2.22 % which is higher than untreated fibers (16.06 ± 0.51 %). Both treatements led to smoother fiber surfaces by removing impurities which confirmed by the Fourier transform infrared (FTIR) analaysis. This demonstrated the ability of B. cereus to degrade the lignocellusic materials in plant fibers, which may used as an alternative to chemical treatments in fiber extraction and textile manufacturing. To optimize the bio-retting process, further research must be done by exploring different parameters such as retting duration, and extracellular enzyme production and fermentation conditions to enhance the efficiency of lignocellulose degradation. Maglangit, Fleurdeliz F. Thesis adviser. UP UP Digital format UPCEB CEBU-LAHUG LG 993 2023 B4 A87 Thesis