00000nam a22000004a 4500 UP-8027390931311922361 Buklod 20250522142031.0 m |o d | cr ||||||||||| 250522s2024 ph r |||| u|eng d UP CPH rda eng LG 993 2024 P85 B55 Billoso, Jannoell Jules B. author Detection and characterization of Acinetobacter baumannii complex and other Gram-negative bacteria in fomites and hands of employees of selected barangay health center in the City of Pasay Jannoel Jules B. Billoso, Larah Alexandria G. Badiola, Louise Bernadine S. Decio, Janine Pauline G. Millan, Clarice Jean Ds. Panday; Sharon Yvette Angelina M. Villanueva, MMSc, PhD, RMicro, DPAM, adviser. 2024 Manila College of Public Health, University of the Phillipines Manila colored illustrations xxii, 381 leaves 28 cm. text rdacontent unmediated rdamedia volume rdacarrier Includes list of acronyms, references and appendices. Special Study Bachelor of Science in Public Health College of Public Health, University of the Philippines Manila 2024 Background: Acinetobacter baumannii complex is a major global concern, especially for nosocomial infections. It presents significant challenges in hospital settings due to its ability to survive and persist in a wide range of environmental conditions, including hands and fomites. While the presence of A. baumannii complex in hospitals is well-established, its prevalence in primary care centers, such as barangay health centers (BHCs) in the Philippines, is less understood. This study serve as a baseline study aimed at determining the proportion of fomites and hands of employees contaminated with A. baumannii complex and other Gram-negative bacteria in a selected BHC in the City of Pasay. It also aimed to determine if the A. baumannii complex isolates from the fomites and hands of employees exhibit antimicrobial resistance (AMR) properties. Materials and Methods: The study used a quantitative descriptive cross-sectional study design to determine the proportion of A. baumanni complex contamination and characterize its phenotypic, biochemical, and molecular characteristics, particularly the presence of antibiotic resistance genes (ARG), and its coexistence with other Gram-negative bacteria in fomite and hand samples from employees of a selected BHC in Pasay City. Samples were cultured on CHROMagar™ and MacConkey agar, and bacterial identity and ARG were confirmed through Gram staining, biochemical tests, and PCR tests targeting blaOXA-51-like, blaOXA-23-like, and ISAba1 genes. Results: Samples were collected from 18 hands of BHC employees and 31 fomites in the study site. Results showed that there was contamination of A. baumannii complex in 66.67% (12/18) of hand samples and 54.84% (17/31) of fomite samples based on their growth in CHROMagar™. Among these, 75.00% (9/12) of hand samples and 35.29% (6/17) of fomite samples tested positive for the blaOXA-51-like gene, indicating that the samples were presumptive A. baumannii species. As for the presence of ARGs, out of the 49 samples tested for the blaOXA-23-like gene, only one (1) tested positive, attributed to a door knob in the nurses' and midwives' office. On the contrary, none of the samples tested for the ISAba1 insertion sequence yielded a positive result. Conclusion: The high contamination rate of A. baumannii complex on fomites and hands in the selected BHC present the likelihood of transmission of A. baumannii complex with the BHC and among the employees, posing exposure and health risks to patients, visitors and employees. This underscores the need for enhanced contamination control practices, such as regular disinfection of health facilities, strict hand hygiene protocols, and increased awareness of microbial contamination. These findings provide a baseline for further public health measures aimed at mitigating community-acquired infections and AMR risks in community healthcare settings. Acinetobacter baumannii Identification. Gram-negative bacteria Identification. Medical personnel Hand hygiene Philippines Pasay City. Health centers Philippines Pasay City. Fomites Microbiology. Badiola, Larah Alexandria G. author. Decio, Louise Bernadine S. author. Millan, Janine Pauline G. author. Panday, Clarice Jean Ds. author. Villanueva, Sharon Yvette Angelina M. adviser. FI UP Print UPMNL CPH LG 993 2024 P85 B55 Thesis