00000nam a22000004a 4500 UP-8027390931311306004 Buklod 20241114152341.0 m |o d | ta 241017s xxu r |||| u|eng d PHARM rda eng LG 993.5 2019 B38 Bautista, Carmina Mae S. author Determination of hypoglycenic effect and cytotoxicity of manihot esculenta crantz (fam. euphorbiaceae) aqueous leaf extract abd its fractions in alloxan-induced diabetic mice Carmina Mae S. Bautista ; Bryan M. Amante ; Edwin Ruamero Jr, . ix, 75 leaves ; 29 cm text rdacontent unmediated rdamedia volume rdacarrier Thesis (Bachelor of Science in Pharmacy)--University of the Philippines Manila, June 2019. Available only after consultation with author /thesis adviser. Available only to those bound by confidentiality agreement Manihot esculent (Crantz) leaves have been used for management diabetes based on cited ethnopharmacological studies. However, scientific evidence to support its efficacy is lacking. The aim of the study was to assess both the in vivo hypoglycemic effect and cytotoxicity of aqueous leaf extract and fractions of the plant in male swiss albino mice. The hypoglycemic activity was examined in alloxan-induced diabetic mice. Cytotoxicity was sereened using CytoTox 96* (Promega), a NonRadioactive Cytotoxicity Assay on liver cancer (HepG2) and normal kidney (HK-2) cell lines. Phytochemical screening identified the composition of the crude extract and its fractions. The aqueous crude extract at dose levels 200 mg/kg and 300 mg/kg body weight showed significant reduction in blood glucose compared to the diabetic control but not exceeding the results of metformin treatment. Five samples were tagged as highly cytotoxic and were flagged for further assays. These samples are: DCM fraction (100 ppm), n- Hexane fraction (1 and 10 ppm), Ethyl acetate fraction (1 ppm), and Aqueous fraction (100 ppm). The phytochemical results showed the presence of alkaloids, anthraquinone glycosides, carbohydrates, phenolic glycosides, saponins, and flavonoids. The effectiveness of crude extract in reduction of blood glucose in mice may be attributed to the synergistic effects of phytochemicals present, especially the alkaloids which were retained in the extract but were undetectable in the fractionated samples. The results of this study also support the findings of existing ethnopharmacological studies on M. esculenta leaf extract as a hypoglycemic agent. Manihot esculenta Hypoglycemic effects Cytotoxicity Diabetic mice. Aqueous extracts Cytotoxicity Diabetes Philippines. Amante, Bryan M. author. Ruamero, Edwin Jr. adviser. FI UPMNL PHARM LG 993.5 2019 B38 Thesis