00000nam a22000004a 4500 UP-8027258850631504462 Buklod 20240816113809.0 m |o d | ta 240816s2024 xxu r |||| u|eng d PHARM rda eng LG 993.5 2024 P5 C38 Catalogo, Cherika Euyce C. author Nanoencapsulation of bixin using ionic gelation method: factors that affect particles attributes Cherika Euyce C. Catalogo, Danielle Justinne B. Clemente, Shanna Nicole A. Ong ; Buenvenido S. Balotro, adviser. Manila: Department of Pharmacy, College of Pharmacy, University of the Philippines Manila 2024 viii, 56, F-3 leaves ; 29 cm. text rdacontent unmediated rdamedia volume rdacarrier Thesis (Bachelor of Science in Pharmaceutical Sciences)--University of the Philippines Manila, June 2024 available to general public Bixin is a carotenoid pigment found in the outer layer seeds of B. orellana found to display multiple biological activities, some of the more prominent ones being its anti-inflammatory and antioxidant properties. Even with these promising potentials, formulating with Bixin has posed to be challenging due to its issues with solubility, and the stability of its nanoformulation in the dispersion system. To overcome these problems, formulations of bixin-loaded chitosan-tripolyphosphate hyaluronic acid-coated nanoparticles (HA-Bx-CSS/TPP NPs) have been produced using ionic gelation to improve its stability in the dispersion system preventing aggregation. Factors such as Bixin Concentration, Chitosan:Tripolyphosphate ratio (CS:TPP), and Hyaluronic Acid (HA) concentration have been explored to determine their effects on the characteristics of the HA-Bx-CSS/TPP nanoparticles through the analysis of Particle Size, Zeta Potential, and Encapsulation Efficiency. Preliminary tests have also been conducted to obtain the maximum solubility of 40% Bixin which is 12mg/mL, and set the appropriate levels for each factor. Each factor was set with five (5) different levels, and each level was used in the formulation. It was found that the Bixin levels three (3) and four (4), with 9 mg/mL and 10 mg/mL , CS:TPP levels (3) 4:1 and (4) 4.25:1, HA levels (2) 0.3% and (3) 0.5% are the best levels for formulation since they resulted in a nanoparticle suspension with no aggregation, <1000 nm size, acceptable PDI, and zeta potential ≥ +/-30mV. Further optimization has been performed and it was found that concentrations of Bixin 9 mg/mL and 10.5 mg/mL in combination with CS:TPP 4:1 and HA 0.3% are the best formulations with %EE 45.54% and 45.20%, respectively. This indicates that Bixin has been incorporated into the nanoparticle. vii bixin Encapsulation. Particle Size. Measurement. Nanoencapsulation Method. Ionic interactions . Clemente, Danielle Justinne B. author. Ong, Shanna Nicole A. author . Balotro, Buenvenido S. adviser. FI UP UPMNL PHARM LG 993.5 2024 P5 C38 Thesis