00000nam a22000004a 4500 UP-1686042739784840207 Buklod 20240816114226.0 m |o d | ta 240408s2023 xxu r |||| u|eng d PHARM rda eng LG 993.5 2023 P5 C85 Gustillo, Stella Rowee author In vitro assessment of the inhibitory effect of Chamaecostus cuspidatus herbal preparation on CYP2C9 Stella Rowee Gustillo ; Joana J. Arejola, adviser . Manila: Department of Pharmacy, College of Pharmacy, University of the Philippines Manila 2023 xxi, 213 leaves ; 28 cm. text rdacontent rdamedia unmediated volume rdacarrier Thesis (Master of Science in Pharmacy)--University of the Philippines Manila, August 2023. Available only after consultation with author/thesis adviser Cytochrome P450 enzyme-mediated herb-drug interactions may pose considerable risk for critical clinical consequences when co-administered with prescribed medications. Oral hypoglycemic agents such as sulfonylureas, meglitinides, and thiazolidinediones are substrates of the most abundant CYP2C subfamily in the human liver-CYP2C9. Concomitant intake with herbal preparations which contain phytoconstituents that may potentially modulate drug metabolism could alter plasma drug concentrations, degree of drug efficacy and toxicity. Chamaecostus cuspidatus leaves traditional and commercial herbal preparations have been taken for diabetes mellitus in Southeast Asia with reports on combined use with allopathic medicine. The investigation of the risk of possible fatal outcomes such severe hypoglycemia through initial in vitro assessment of potential CYP2C9 inhibition by natural products may be of great importance for treatment optimization. The study assessed the ability of traditionally and commercially prepared Chamaecostus cuspidatus aqueous extracts to interfere with CYP2C9 activity via fluorescence-based enzyme assay. Among the aqueous extracts of Chamaecostus cuspidatus leaves, only the lyophilized leaf juice from Bacolod showed potential CYP2C9 inhibition. Flavonoids and alkaloids were detected via TLC and phytochemical screening which are the most likely class of metabolites in Chamaecostus cuspidatus responsible for the CYP2C9 inhibitory activity. Although none of the decoctions and tea preparations showed significant CYP2C9 percentage inhibition, further evidence is needed to determine the effect of heating on the CYP2C9-inhibiting compounds from Chamaecostus cuspidatus. Cytochrome P-450 Enzyme System. Drug Interactions. Herbal Medicine. Hypoglycemic agents. Inhibition. Diabetes Mellitus. Chamaecostus cuspidatus. Cytochrome P450. Herb-Drug Interactions. Traditional Medicine. Phytoconstituents. Commercial Herbal Preparations. Arejola, Joana J. adviser. FI UP UPMNL PHARM LG 993.5 2023 P5 C85 Thesis