00000nam a22000008i 4500 UP-1685675941131534216 Buklod 20231130150600.0 m |o d | cr ||||||||||| 231106s2021 nju r b|001 0|eng d 2020029236 9781119640783 (paperback) 21595021 DNLM/DLC eng rda DLC CASM eng QH442.2 B87 2021 Brown, T. A. (Terence A.) author. Gene cloning and DNA analysis an introduction Professor Terry A. Brown. Eighth edition. 2010 Hoboken, NJ Wiley-Blackwell 2021. 416 pages 24cm colored illustrations text txt rdacontent unmediated rdamedia volume nc rdacarrier Includes bibliographical references and index. Why gene cloning and DNA analysis are important -- Vectors for gene cloning : plasmids and bacteriophages -- Purification of DNA from living cells -- Manipulation of purified DNA -- Introduction of DNA into living cells -- Cloning vectors for Escherichia coli -- Cloning vectors for eukaryotes -- How to obtain a clone of a specific gene -- The polymerase chain reaction -- Sequencing genes and genomes -- Studying gene expression and function -- Studying genomes -- Studying transcriptomes and proteomes -- Production of protein from cloned genes -- Gene cloning and DNA analysis in medicine -- Gene cloning and DNA analysis in agriculture -- Gene cloning and DNA analysis in forensic science and archaeology. "The main development in DNA technology since publication of the Seventh Edition of Gene Cloning has been the increased use of gene editing as a tool in both research and biotechnology. The basic methodology for CRISPR editing is now described in Chapter 12 and the applications of the method are explored, in the context of plant genetic engineering, in Chapter 16. Elsewhere, the continuing evolution of next-generation DNA sequencing is reflected by a reorganization of this part of Chapter 10, and to deal with the further proliferation of methods for studying transcriptomes and proteomes (albeit not strictly DNA Analysis) I have created a new chapter devoted to these methods. Other additions include new sections on melt curve analysis of real-time PCR products and genetic typing of human disease mutations"-- Provided by publisher. Cloning, Molecular. Sequence Analysis, DNA. DNA, Recombinant analysis. Online version Brown, T. A. (Terence A.) Gene cloning and DNA analysis Eighth edition. Hoboken, NJ : Wiley-Blackwell, 2021. 9781119640752 (DLC) 2020029237. FO UPMNL CASM QH 442.2 B87 2021 Book