00000nam a22000004a 4500 UP-1685675941131387508 Buklod 20240104124847.0 m |o d | ta 240104s2023 xx r |||| u| d PHARM rda eng LG 993.5 2023 P5 B35 Baliton, Rebecca Aira S. author Development of thin layer of chromatography method for the profiling of cymbopogon citratus (D.C) stapf. Rebecca Aira S. Baliton, Bernadine C. Comendador, James Christian F. Tan ; adviser, Joanna J. Orejola. Manila Department of Pharmacy, College of Pharmacy, University of the Philippines Manila 2023 ix, 128 leaves 28 cm. text rdacontent unmediated rdamedia volume rdacarrier Thesis (Bachelor of Science in Pharmaceutical Sciences)--University of the Philippines Manila, June 2023. Available to the public. Cymbopogon citratus (DC.) Stapf. is widely used in traditional medicine for its various bioactivities, as well as in modern commodities (Oladeji et al., 2019). However, there is a gap in the methods that can be used for the assessment of C. citratus non-essential oil extracts. This study aims to develop a thin layer chromatography method for the profiling of C. citratus methanolic extract. The method development and optimization focused on the chromatographic conditions based on sample concentration, mobile phase, chromatographic chamber, equilibration time, and derivatization. The profile of C. citratus samples from different locations and extract preparations were also assessed to come up with a TLC profile. The specificity of the method was also determined using other plant species namely, Citronella (Cymbopogon nardus or Cymbopogon winterianus), Paragis (Eleusine indica), and Plant X (identified to be lemongrass). As a result, the developed method requires a 10 pg/band Ioading amount of the lyophilized C. citratus extract. The mobile phase consists of Toluene : Ethyl Acetate : Glacial Acetic Acid (80:18:2 v/v/v) using either Camag® 20x20 twin trough chamber (30 mL) or a cylindrical makeshift 6x13 chamber (6 mL). The ideal equilibration time is at least 15 minutes from full chamber saturation up to 3 hours. Derivatization of the plates can be done using vanillin/sulfuric acid and ammonium molybdate spray reagent under white light and 366 nm, respectively. The possible chemical markers for drying, degradation, and if a sample is prepared fresh for C. citratus were proposed as well as other markers for Poaceae. These markers can be identified and isolated to establish chemical markers for the thin layer chromatography of C. citratus. Thin layer chromatography. Development Cymbopogon citratus. Chromatography--methods. Comendador, Bernadine C. author. Tan, James Christian F. author. Orejola, Joanna J. adviser. FI UP UPMNL PHARM LG 993.5 2023 P5 B35 Thesis