00000ctmaa22000003a 4500 UP-1685675941123958186 Buklod 20180816141714.0 aa rb |||1 u| ta 180816s xx d r |||| u| (iLib)UPTAC-00019010305 UPVTC eng LG 993.5 2018 B5 G336 Gadores, Mc Colleine S. author. Phenotypic-based screening of the antimelanogenic properties of selected mangrove methanolic crude extracts using zebrafish (Damio rerio) as model. Mc Colleine S. Gadores 2018 x, 33 leaves illustrations. Undergraduate thesis (B.S. Biology) -- University of the Philippines, Tacloban. Due to abnormal increase in the production and accumulation of melanin, researches in relation to the alteration of skin pigmentation have been in focus by researches. As an outcome many known substances such as kojic acid, and arbutin have been used to reduce the level of pigmentation. Though effective, some exhibits poor skin penetration and side effects ranging from a common allergic reactions to mutation. Thus, researchers have been looking for alternative sources of potent anti-melanogenic compounds. In this study, methanolic crude extracts from Avicennia alba, Avicennia offcinalis, Camptostemon philippinensis, Lumnitzera racemose, and Pemphis acidula were screened for their anti-melanogenic properties using zebrafish as a model organism. Screening of these plant extracts was done by applying 50 ug/mL and 100 ug/mL concentration of each extracts during their 9 hours post fertilization (hpf) and were observed at 55 hpf. Results showed that some extracts were toxic to the larvae and that the extracts exhibited none to profound effects on the pigmentation of the larvae. And as shown in the results, C. philippinensis exhibited the best results in terms of its effect on melanogenesis and has a profound effect to the entire population. It was also observed that although its toxicity increases as concentration is increased, its effect on melanogenesis also increases and as shown by ImageJ and One-way ANOVA, 200 ug/mL of the extract showed almost to no significant difference as the effect of kojic acid used as a control. Further isolation and purification of the active compound present in the extract could lead to the discovery to a more potent and effective anti-melanogenic compound. Fuentes, Rolly G. adviser. FI UP UPTAC UPTAC LG 993.5 2018 B5 G336 Thesis