00000nam a22000004a 4500 UP-1685675941122084263 Buklod 20240126134856.0 a r |||| u| ta 240126s2023 xx grbm 00| 0 eng d DCHE rda eng DMLUC LG 993.5 2023 F66 M55 Milanay, Ian Lemuel P. author. Preliminary identification of allergens in pectobacterium phage lyases for soft rot pectobacteriaciae biocontrol in silico approach Ian Lemuel P. Milanay. Quezon City College of Home Economics, University of the Philippines Diliman 2023. xii, 54 leaves illustrations 28 cm. text rdacontent unmediated rdamedia volume rdacarrier Thesis Bachelor of Science in Food Technology University of the Philippines Diliman 2023. Includes bibliographical references (pages 35-46) Thesis classification: F The persistence of bacterial soft rot disease extensively affected local farming communities and demanded more potent mitigation that is both economical and efficient. Bacteriophages were deemed to suit these requirements as biocontrol agents, however, their allergenicity for human consumption had not been substantiated. This study analyzed the allergen potential of six lyases from Pectobacterium phage Arno160, vB_PatP_CB4, PP101, and vB_PcaM_CBB through a four-point strategy using five web-based in silico programs to provide baseline data and satisfy the recommendations of WHO-IUIS. It was determined that an aa fragment from vB_PcaM_CBB (1) lyase is a Class 1 allergen with 48% identity and 78% similarity to Avenin-F and may illicit oral allergy syndrome in patients with coeliac disease. The lyases from Arno160, as well as the vB_PcaM_CBB (2,3), were also found to be probable allergen, which requires in sera examination for confirmatory validation. The lyases from PP101 and vB_PatP_CB4 rendered no positive hits from the programs and were ruled out as probable non-allergen, thereby suggesting their potential use as biocontrol candidates in future Next Generation Risk Assessment studies. Allergens Philippines. Bacteriophages Philippines. Bioinformatics Philippines. Phytopathogenic microorganisms Biological control Philippines. Tolentino, Mark paulo S. adviser. FI UP UPD DCHE LG 993.5 2023 F66 M55 Thesis