00000nam a22000004a 4500 UP-1685675941122043876 Buklod 20240126102129.0 a r |||| u| ta 240126s2023 xx grbm 00| 0 d DCHE rda eng DMLUC LG 993.5 2023 F66 A55 Ang, Nadia Martina B. author. In silico screening of potential bioactive peptides in milkfish (Chanos chanos) muscle proteins Nadia Martina B. Ang. Quezon City College of Home Economics, University of the Philippines Diliman 2023 x, 135 leaves illustrations 28 cm. text rdacontent unmediated rdamedia volume rdacarrier Thesis Bachelor of Science in Food Technology University of the Philippines Diliman 2023. Includes bibliographical references (pages 57-70) Thesis classification: F Identification and isolation of bioactive peptides from fish sources is an emerging research area for their potential use as ingredients in functional foods and nutraceuticals. Bioinformatics tools provide a more cost-effective and time saving method for predicting potential bioactivities in proteins and aiding experimental methods by guiding enzyme selection for producing fish protein hydrolysates (FPHs). Thus, this study used the in silico tool BIOPEP-Uniwesytet Warmińsko-Mazurski (BIOPEP-UWM) to evaluate the potential of ten representative milkfish (Chanos chanos) muscle proteins to release bioactive peptides with 12 proteases and two enzyme combinations. Results revealed that milkfish is a potentially rich source of antioxidant and angiotensin-converting-enzyme (ACE) inhibiting peptides. Pepsin, papain, and bromelain were most effective in liberating ACE inhibitors while proteinase K, pepsin, and ficin were most successful in releasing antioxidant peptides. Combinations of gastrointestinal (GI) enzymes were also shown to release large amounts of both ACE inhibitors and antioxidants. Furthermore, using PeptideRanker, 40 novel peptides were identified after proteolysis. Additional bioinformatic tools like AllerTop, PeptideCutter, ToxinPred, AHTPin, and AnOxPePred were also used in the study to characterize selected novel peptides on their allergenicity, resistance to GI digestion, toxicity, and predicted antihypertensive and antioxidant activities. The present work suggests that C. chanos is a good source of ACE inhibiting and antioxidant peptides and that in silico approaches can serve as theoretical basis for future in vitro and in vivo research on bioactive peptides from C. chanos proteins. Milkfish Philippines. Fishery processing Philippines. Food Philippines. Sanchez, Rowena Grace R. adviser. FI UP UPD DCHE LG 993.5 2023 F66 A55 Thesis