00000caa a22000003i 4500 UP-1685594773862409357 Buklod 20210610175049.0 m | | ta 210610s2020 xx r |||| u|eng d (iLib)UPBAG-00040898157 STII-DOST BAG rda eng ARTICLE-3538 Miyar, Harshitha Kamath author. Extracellular polymeric substance produced by Lysinibacillus sp. SS1 in response to petroleum crude oil stress facilitates removal of heavy metals from aqueous solutions by Harshitha Kamath Miyar, Annapoorna Pai, Shyama Prasad Sajankila, and Louella Concepta Goveas. 2020. pages 1207-1215 color illustrations 26 cm text rdacontent unmediated rdamedia volume rdacarrier Includes bibliographical references (pages 1213-1215) TThe use of microbial extracellular polymeric substances (EPS) as bioflocculants opposed to synthetic and organic flocculants in the treatment of heavy metal polluted wastewater is currently gaining importance. The present study focuses on the characterization and application of EPS produced by Lysinibacillus sp. SS1 in heavy metal removal. Lysinibacillus sp. SS1 (10%v/v), isolated from used engine oil contaminated soil, produced EPS and circular white spongy masses on the utilization of 3% petroleum crude oil (PCO) in the Bushnell Haas medium in 10 d. Analysis of extracted EPS and masses revealed that it contained carbohydrates, lipids, and proteins in proportions of 1.3: 94.6: 3.6 and 2.3: 91.5: 6.2, respectively. Characterization by LCMS and FT-IR analysis confirmed that they were predominantly lipids with proteins and polysaccharide groups. Masses could remove 48% of lead and 40% of mercury from aqueous solutions by adsorption on their surface as confirmed by SEM-EDAX. Extracted EPS (100 mg/L) could remove 94.9% of lead from aqueous solution (150 mg/L) in 72 h by precipitation of lead as nanoparticles of sizes ranging from 30?40 nm. This study shows the potential of Lysinibacillus sp. SS1 and its EPS in response to PCO in heavy metal removal and recovery. Extracellular polymeric substance. Lead. Lysinibacillus sp. SS1. Mercury. Nanoparticles. Petroleum crude oil. Pai, Annapoorna author. Sajankila, Shyama Prasad author. Goveas, Louella Concepta author. The Philippine Journal of Science Vol. 149, no. 4, December 2020. Request full-text access via UPB University Library through https://forms.gle/KZjBv7aRtY6jiL5E9 (viewed 10 June 2021) FI UPBAG UPBAG-MAIN ARTICLE-3538 Analytics