00000caa a22000003i 4500 UP-1685594773862409356 Buklod 20210610173941.0 m | | ta 210610s2020 xx r |||| u|eng d (iLib)UPBAG-00040898156 STII-DOST BAG rda eng ARTICLE-3537 Ragudo, Gabriel Phillip author. RAD51 135G>C single nucleotide polymorphism and risk of breast cancer in selected Filipino cases by Gabriel Phillip Ragudo, Beatriz Soneth Pureza, Denise Anne Rimando, Mikael Remus Manrique, Teresa Sy Ortin, Kin Israel Notarte, and Pia Marie Albano. 2020. pages 1199-1206 26 cm text rdacontent unmediated rdamedia volume rdacarrier Includes bibliographical references (pages 1204-1205) The RAD51 gene encodes the protein that plays a central role in the repair of DNA double-strand breaks (DSBs) through the homologous recombination pathway. Association of RAD51 single nucleotide genetic polymorphism (SNP) with the development of cancer has been observed to be tumor site- and race-specific. Thus, this study aimed to determine the potential association between RAD51 135G>C SNP and breast cancer among selected Filipinos. Patients with histologically confirmed breast cancer (n = 60) seen at the University of Santo Tomas Hospital (USTH Manila) were age- and sex-matched with clinically healthy controls (n = 60). Genomic DNA was extracted from the blood of participants and analyzed for RAD51 genotype by polymerase chain reaction ? restriction enzyme fragment length polymorphism (PCR-RFLP). A significantly higher incidence of RAD51 C/C genotype was seen among the cases than the controls (p < 0.05). The more common G/C genotype was not associated with breast cancer development, while the recessive less common C/C genotype was observed to potentially increase the risk. However, passive smokers carrying the RAD51 G/C genotype had a significantly increased chance of developing breast cancer. RAD51 G/C genotype ? even when combined with other established risk factors like alcohol use, active smoking, and family history ? were not associated with breast cancer. BRCA genes. Breast cancer. Filipinos. Passive smoking. PCR-RFLP. RAD51 135G>C. Pureza, Beatriz Soneth author. Rimando, Denise Anne author. Manrique, Mikael Remus author. Ortin, Teresa Sy author. Notarte, Kin Israel author. Albano, Pia Marie uathor. The Philippine Journal of Science Vol. 149, no. 4, December 2020. Request full-text access via UPB University Library through https://forms.gle/KZjBv7aRtY6jiL5E9 (viewed 10 June 2021) FI UPBAG UPBAG-MAIN ARTICLE-3537 Analytics