00000caa a22000003i 4500 UP-1685594773862409350 Buklod 20210610162718.0 m | | ta 210610s2020 xx r |||| u|eng d (iLib)UPBAG-00040898150 STII-DOST BAG rda eng ARTICLE-3532 Sapin, Arsenia B. author. Response surface methodology guided optimization of the extraction of natural phenolics from mango (Mangifera indica Linn) branches by Arsenia B. Sapin, Rodney H. Perez, Fides Marciana Z. Tambalo, Arra Gaylon, and Maria Katrina N. Alaon. 2020. pages 1147-1156 color illustrations 26 cm text rdacontent unmediated rdamedia volume rdacarrier Includes bibliographical references (pages 1155-1156) Phenolic compounds are important compounds that are known for their antioxidant bioactivities and other health-promoting properties. Different parts of mango (Mangifera indica Linn) tree,including their branches, are known to contain polyphenols. Mango branches that are cut off during pruning have no significant economic value. Polyphenols from mango branches can potentially provide an alternative source of income to farmers during non-fruiting season. In this study, response surface methodology (RSM) was used to identify the optimum conditions for the solvent-assisted extraction of phenolics from mango branches. Optimization conditions for two different solvents, acetone and ethanol, were done. A Box-Behnken experimental design (BBD) identified solids loading, solvent concentration, and extraction time as factors that significantly affected the total polyphenol (TP) extraction. The optimum conditions for acetone-assisted extraction based on RSM analysis were as follows: 15% w/v-solids loading, 60% v/v-solvent concentration, and 1.5-h extraction time. The optimum conditions for ethanol-assisted extraction were as follows: 14.858% w/v-solids loading, 59.535% v/v-solvent concentration, and 1.763-h extraction time. The predicted TP values of acetone and ethanol extract using these optimum conditions were 32.7143 mg gallic acid equivalents (GAE) and 35.8963 mg GAE, respectively. The actual TP values when the optimum conditions were used in an extraction, which were 32.0358 mg GAE for acetone extract and 33.0075 mg GAE for ethanol extract, were within the 95% prediction interval making the optimization points valid. HPLC (high-performance liquid chromatography) analysis of the phenolic extract showed that mangiferin was the main phenolic compound in mango branches. Mango. Mango branches. Optimization. Phenolics. Response surface methodology. Solvent extraction. Perez, Rodney H. author. Tambalo, Arra Gaylon author. Alaon, Maria Katrina N. author. The Philippine Journal of Science Vol. 149, no. 4, December 2020. Request full-text access via UPB University Library through https://forms.gle/KZjBv7aRtY6jiL5E9 (viewed 10 June 2021) FI UPBAG UPBAG-MAIN ARTICLE-3532 Analytics