00000caa a22000003i 4500 UP-1685594773862408712 Buklod 20210408171333.0 m | | ta 210408s2020 xx r |||| u|eng d (iLib)UPBAG-00039679874 STII-DOST BAG rda eng ARTICLE-3038 Mabazza, Kristel Anna A. author. Steam explosion and sequential steam explosion - dilute acid pretreatment optimization of banana pseudostem for polyhydroxybutyrate (PHB) production by Kristel Anna A. Mabazza, Princess J. Requiso, Catalino G. Alfafara, Fidel Rey P. Nayve Jr., and Jey-R S. Ventura. 2020. pages 287-297 color illustrations 26 cm text rdacontent unmediated rdamedia volume rdacarrier Includes bibliographical references (pages 295-297) Polyhydroxybutyrate (PHB) is a suitable biodegradable alternative to non-renewable petroleum-based plastics. Readily available agricultural lignocellulosic residues such as banana pseudostem can be utilized as a substrate to reduce the production cost of PHB and improve its feasibility for commercialization. Accordingly, pretreatment of banana pseudostem is needed to efficiently convert the substrate to PHB. In this study, optimization of two pretreatment methods for banana pseudostem ? namely, steam explosion and sequential steam explosion ? dilute acid pretreatment ? were optimized to improve the digestibility of the biomass and consequently increase the production of reducing sugars in the hydrolysate during enzymatic saccharification. Response surface methodology (RSM)-designed experiments showed that among all factors investigated, for both pretreatment methods, the steam explosion temperature had the strongest positive impact on reducing sugar production. Optimum conditions of steam explosion pretreatment were 219.31 °C steam explosion temperature and 10 min of pretreatment time, producing 7.33 g/L (48.87% yield) of reducing sugars in the enzymatic hydrolysate. For sequential pretreatment, optimum conditions were 220 °C steam explosion temperature, 135 °C dilute acid temperature, 44 min of dilute acid reaction time, and 1.57% w/v H2SO4, with a corresponding reducing sugar concentration of 13.02 g/L (86.79% yield). A 90% increase in reducing sugar yield was observed after dilute acid pretreatment of steam-exploded banana pseudostem. Using the hydrolysate from sequentially-pretreated banana pseudostem, PHB (2.64 g/L) was successfully synthesized after 12 h of bacterial fermentation. Hence, sequential pretreatment was proven effective in producing enzymatic hydrolysates from banana pseudostem for PHB production. (Author's abstract) Chemistry. Banana pseudostem. Enzymatic saccharification. Polyhydroxybutyrate. Response surface methodology. Sequential steam explosion dilute acid pretreatment. Steam explosion. Ventura, Jey-R S. author. Nayve, Jr., Fidel Rey P. author. Requiso, Princess J. author. Alfafara, Catalino G. author. The Philippine Journal of Science Vol. 149, no. 2, June 2020. Request full-text access via UPB University Library through https://forms.gle/KZjBv7aRtY6jiL5E9 (viewed 08 April 2021) FI UPBAG UPBAG-MAIN ARTICLE-3038 Analytics