00000caa a22000003i 4500 UP-1685594773862408672 Buklod 20210407201333.0 m | | ta 210407s2020 xx r |||| u|eng d (iLib)UPBAG-00039679833 STII-DOST BAG rda eng ARTICLE-3002 Sangel, Percival P. author. Importin-β proteins expression modulation in embryonic stem cells and embryonic fibroblasts of mouse by Percival P. Sangel. 2020. pages 21-26 color illustrations 26 cm text rdacontent unmediated rdamedia volume rdacarrier Includes bibliographical references (page 26) Importin-β proteins are transport proteins important in the shuttling of cargo proteins by binding to either nuclear localization signal (NLS) or nuclear export signal (NES). This study investigated the in vitro expression modulation of selected importin-βs. Specifically, this study characterized the culture behavior of mouse embryonic stem cells after knockdown of selected importin-β proteins like Cse1L, IPO7, KPNB1, RanBP16, RanBP17, or XPO4. Also, this study assessed the effects of overexpressing RanBP17 or IPO7 during cellular reprogramming of mouse embryonic fibroblasts (MEFs). Results showed that Cse1L and KPNB1 are essential for the viability of mouse embryonic stem cells since knockdown of either one of these proteins resulted in the death of mouse embryonic stem cells. Meanwhile, the growth characteristics of RanBP17, XPO4, IPO7, or RanBP16 knockdown mouse embryonic stem cells were comparable with the control. Aside from round colonies, the appearance of flat cells and spreading growth characteristics in some colonies were observed, which indicated early signs of differentiation. On the other hand, the number of colonies with overexpressed Oct4, Sox2, Klf4, cMYC (OSKM) + RanBP17, or OSKM+ IPO7 was comparable to OSKM+Flag or OSKM (controls). This suggests that RanBP17 or IPO7 has limited application in the generation of induced pluripotent stem cells. (Author's abstract) Biology. Cellular reprogramming. Importin-#946 proteins. Knockdown. The Philippine Journal of Science Vol. 149, no. 1, March 2020. Request full-text access via UPB University Library through https://forms.gle/KZjBv7aRtY6jiL5E9 (viewed 07 April 2021) FI UPBAG UPBAG-MAIN ARTICLE-3002 Analytics