00000caa a22000003i 4500 UP-1685594773862408510 Buklod 20210324155002.0 m | | ta 210324s2019 xx r |||| u|eng d (iLib)UPBAG-00039569129 BAG rda eng ARTICLE-2881 Turaja, Kimberly I B. author. Influence and total antioxidant capacity of non-enzymatic antioxidants on the quality and integrity of extended and cryopreserved semen of Murrah buffalo (Bubalus bubalis) by Kimberly I B. Turaja, Renato SA. Vega, Thelma A. Saludes, Abraham G. Tandang, Jose Arceo N. Bautista, Agapita J. Salces, and Carmelita M. Rebancos. 2019. pages 619-626 26 cm text rdacontent unmediated rdamedia volume rdacarrier Includes bibliographical references (pages 616-618) The study compared the effect of non-enzymatic antioxidants on the % motility, livability, and plasma membrane integrity of the cryopreserved sperm from four purebred Murrah bulls aged 10?17 years old. The level of antioxidants was also determined. Experiment 1 was analyzed using 4 x 4 factorial design in a randomized complete block design (RCBD) with bulls blocked in four; in Experiment 2, it analyzed the levels of MegaAntiox using PROC ANOVA of SAS software. Results revealed no significant (P > 0.05) difference in post-thaw sperm motility (%) between antioxidants but a significant decrease (P < 0.05) in the post-thaw sperm motility was observed at Week 3. The addition of non-enzymatic antioxidants did not affect (P > 0.05) the live sperm though difference was observed on the livability of sperm cells in different collection weeks. In extended semen, the addition of non-enzymatic antioxidants did not show any significant effect (P > 0.05) on post-thaw sperm motility (%) and livability. After six months of cryopreservation, the addition of vitamin E (one of the non-enzymatic antioxidants) had a negative effect on the post-thaw sperm motility. Livability and PMI were not significantly affected (P > 0.05) by the different treatments. In conclusion, the addition of 0.1 mg/ml non-enzymatic antioxidants did not improve sperm motility (%) and the livability of the sperm. (Author's abstract) cryopreservation. Murrah buffalo. Non-enzymatic antioxidants. Semen. Semen extender. Vega, Renato SA. author. Saludes, Thelma A. author. Tandang, Abraham G. author. Bautista, Jose Arceo N. author. Salces, Agapita J. author. Rebancos, Carmelita M. author. The Philippine Journal of Science Vol. 148, no. 4, December 2019. Request full-text access via UPB University Library through https://forms.gle/KZjBv7aRtY6jiL5E9 (viewed 24 March 2021) FI UPBAG UPBAG-MAIN ARTICLE-2881 Analytics