00000caa a22000003i 4500 UP-1685594773862388646 Buklod 20210323092305.0 m | | ta 210323s2019 xx r |||| u|eng d (iLib)UPBAG-00039569074 STII-DOST BAG rda eng ARTICLE-2827 Alojado-Rubianes, Ma. Roselette L. author. Digital image photometry of xylose in liquid medium by Ma. Roselette L. Alojado-Rubianes and Ernesto J. del Rosario. 2019. pages 217-224 illustrations 26 cm text rdacontent unmediated rdamedia volume rdacarrier Includes bibliographical references (pages 223-224) Digital image photometry (DIP) was used to determine xylose concentration in liquid medium. Xylose was quantified by its color reaction with phloroglucinol in a 96-well microplate sample holder using a commercial flat-bed scanner followed by image analysis with free-access software (ImageJ). The Red (R) values showed linear correlation versus xylose concentration in the range of 1.0?10.0mg/mL with correlation coefficient of 0.987. The DIP method was found to be accurate based on experimental pentose (versus theoretical) concentration values. The observed limits of detection (LOD) and quantification (LOQ) for xylose were 0.65mg/mL and 2.17mg/mL, respectively. The DIP method gave results that are reliable and comparable to other technical protocols requiring expensive equipment such as high performance liquid chromatograph (HPLC) or UV-visible spectrophotometer. The DIP method was used to evaluate the extent of xylose assimilation in liquid fermentation medium by four yeast strains. (Author's abstract). Chemistry. Digital photometry. Flatbed scanner. Image analysis. Phloroglucinol assay. Xylose determination. del Rosario, Ernesto J. author. The Philippine Journal of Science Vol. 148, no. 2, June 2019. Request full-text access via UPB University Library through https://forms.gle/KZjBv7aRtY6jiL5E9 (viewed 22 March 2021) FI UPBAG UPBAG-MAIN ARTICLE-2827 Analytics