00000caa a22000003i 4500 UP-1685594773862388631 Buklod 20210322211402.0 m | | ta 210322s2019 xx r |||| u|eng d (iLib)UPBAG-00039569059 STII-DOST BAG rda eng ARTICLE-2816 Austria, Eleanor S. author. Vertical structure of heterotrophic bacterioplankton communities in the Western Pacific Ocean by Eleanor S. Austria, Chun-Wei Chang, Ku-Wai Wang, Joan S. Awingan, Kuo-Yuan Li, and Fuh-Kwo Shiah. 2019. pages 155-165 illustrations, map 26 cm text rdacontent unmediated rdamedia volume rdacarrier Includes bibliographical references (pages 163-165) The abundance and composition of summer heterotrophic bacterioplankton communities at the Western Pacific Ocean (WPO) were determined using catalyzed-reporter deposition-fluorescence in situ hybridization (CARD-FISH) technique. Several oligonucleotide probes specific to the Domain Bacteria (EUB338), Phylum Proteobacteria (α-, β-, and γ-Proteobacteria and SAR 11); Actinobacteria (HGC); and Cytophaga-Flavobacter-Bacteroides (CFB) were used to identify and quantify the common bacterial phyla relative to total microbial abundance using 4? 6-diamidino-2-phenylindole (DAPI) over a depth profile?from 10 to 4000m. The profiles of microbial and bacterial abundance, temperature, and oxygen all decreased with depth, in contrast to the profiles of the inorganic compounds. Around 30?59% of the DAPI-positive cells took up the EUB338 probe, suggesting that bacteria are numerically significant components of the microbial biomass in the WPO. Phylum Proteobacteria was the predominant group in the whole water column, with classes α-and γ-Proteobacteria and SAR 11 numerically dominating at specific depths. Statistical analyses showed an association between the bacterial groups?α-Proteobacteria and CFB?and the environmental parameters?temperature and chlorophyll-a concentration?at the depth of 100m, suggesting the role of organic matter and temperature in structuring the bacterial community in this oceanic regime. This study provided a preview on the abundance and controls of specific groups of heterotrophic bacteria in the WPO, warranting a more detailed and comprehensive investigation of the community structure at clade or genus level. (Author's abstract) Biology. card-fish. Community structure. Eub 338. Heterotrophic bacteria. Western pacific ocean. Shiah, Fuh-Kwo author. Awingan, Joan S. author. Li, Kuo-Yuan author. Wang, Ku-Wai author. Chang, Chun-Wei author. The Philippine Journal of Science Vol. 148, no. 1, March 2019. Request full-text access via UPB University Library through https://forms.gle/KZjBv7aRtY6jiL5E9 (viewed 22 March 2021) FI UPBAG UPBAG-MAIN ARTICLE-2816 Analytics