00000caa a22000003i 4500 UP-1685594773862388624 Buklod 20210322184848.0 m | | ta 210322s2019 xx r |||| u|eng d (iLib)UPBAG-00039569052 STII-DOST BAG rda eng ARTICLE-2809 Saracanlao, Rochelle Joie A. author. Influence of different extraction methods on fatty acid composition of lipid extracts of Chlorella vulgaris Beijerinck from Laguna De Bay, Philippines by Rochelle Joie A. Saracanlao, June Owen Nacorda, and Hidelisa P. Hernandez. 2019. pages 95-104 illustrations (some color) 26 cm text rdacontent unmediated rdamedia volume rdacarrier Includes bibliographical references (pages 102-104) Chlorella vulgaris Beijirinck cultures isolated from Laguna de Bay were cultivated and the biomass produced after one month of cultivation was subjected to stepwise selection of solvent system, algae-to-solvent ratio, and extraction method. The optimized procedure for C. vulgaris was found to be sonication with 2:1 (v:v) chloroform:methanol as extractant at 1:20 (g:ml) algae:solvent ratio producing 51% crude lipid (dry-weight basis). Using thin layer chromatography, the C. vulgaris lipid extract showed the following approximate composition: triglycerides (79%), sterols (14%), and 1,3-diglycerides present in substantial amounts. Gas chromatography? flame ionization detection of the fatty acids as fatty acid methyl esters showed the following components: myristic acid, palmitic acid, stearic acid, oleic acid, linoleic acid, linolenic acid, and traces of lauric acid. Analysis of the lipid extracts showed that extraction procedure, along with the solvent system, affects both yield and lipid profile. Total lipid yield and sterol yield increase with increasing polarity of the solvent system. Triglycerides, on the other hand, decreases with the polarity of solvent systems used. A more varied fatty acid class was obtained using more polar solvents (8 fatty acids) compared with the usage of less polar ones (1-3 fatty acids). (Author's abstract) Chemistry. Algal lipids. Fatty acid profile. Lipid yield. Solvent polarity. Solvent polarity. Hernandez, Hidelisa P. author. Nacorda, June Owen author. The Philippine Journal of Science Vol. 148, no. 1, March 2019. Request full-text access via UPB University Library through https://forms.gle/KZjBv7aRtY6jiL5E9 (viewed 22 March 2021) FI UPBAG UPBAG-MAIN ARTICLE-2809 Analytics