00000caa a22000003i 4500 UP-1685594773862388579 Buklod 20210319173830.0 m | | ta 210319s2018 xx r |||| u|eng d (iLib)UPBAG-00039513770 STII-DOST BAG rda eng ARTICLE-2764 Sahagun, Jorge author. Organogenic potential of dendrobium floral tissues for stable transformation applications by Jorge Sahagun, Anupan Kongbangkerd, and Kumrop Ratanasut. 2018. pages 667-676 color illustrations 26 cm text rdacontent unmediated rdamedia volume rdacarrier Includes bibliographical references (page 675-676) This study aimed to examine a promising procedure for in vitro direct organogenesis from floral tissues of Dendrobium Sonia ?Earsakul? as a new potential regeneration system for transformation in orchids. Petal and sepal explants from floral buds (2.0?2.3 cm) survived on ½ MS solid medium supplemented with or without 1-naphthaleneacetic acid (NAA) or/and benzylaminopurine (BA), but only petal explants generated protruding meristemoid tissues when they were cultured on ½ MS medium supplemented with both NAA and BA. A significant increase in the number of protruding meristemoid tissues was observed in the liquid medium. The ½ MS liquid medium supplemented with 0.5 mg L?1 NAA and 1.0 mg L?1 BA was successfully used to induce meristemoids of petal tissues transiently transformed by A. tumefaciens strain EHA105 carrying pCAMBIA?1301 via agroinfiltration and the subsequent use of 20 mg L?1 meropenem was effective in eliminating Agrobacterium from the infected explants. The transformed status of the meristemoid tissues was confirmed by GUS expression analysis, indicating that the T-DNA of the vector used had been successfully incorporated into the genome. For the first time, the researchers have demonstrated the potential methodology for direct organogenesis from agroinfiltration-transformed petal tissues of D. Sonia ?Earsakul?. Agriculture. Dendrobium. Floral tissue. Meristemoid. Organogenesis. Tissue culture. Transformation. Kongbangkerd, Anupan author. Ratanasut, Kumrop author. The Philippine Journal of Science Vol. 147, no. 4, December 2018. Request full-text access via UPB University Library through https://forms.gle/KZjBv7aRtY6jiL5E9 (viewed 19 March 2021) FI UPBAG UPBAG-MAIN ARTICLE-2764 Analytics