00000caa a22000003i 4500 UP-1685594773862388577 Buklod 20210319171206.0 m | | ta 210319s2018 xx r |||| u|eng d (iLib)UPBAG-00039513768 STII-DOST BAG rda eng ARTICLE-2762 Rellin, Klidel Fae B. author. Untargeted metabolite profiling of Philippine-grown crescentia cujete and its commercial fruit juice using GC-MS and UPLC-HRMS by Klidel Fae B. Rellin, Dianne D. Dasmariñas, and Hiyas A. Junio. 2018. pages 647-658 color illustrations 26 cm text rdacontent unmediated rdamedia volume rdacarrier Includes bibliographical references (pages 657-658) Calabash fruit (Crescentia cujete L.) juice has gained traction in Southern Philippines for its miraculous effect against stroke, diabetes, and cancer. Metabolite profile of local C. cujete fruit pulp and the commercial fruit juice were established using gas chromatography mass spectrometry (GC-MS) and ultra-high perfromance liquid chromatography high-resolution mass spectrometry (UPLC-HRMS). Putative hits to the NIST Mass Spectral Library included methyl salicylate, (+)-δ-cadinene, benzene and its derivatives such as toluene and o-xylene. Toluene and o-xylene are known air pollutants. Results indicated that the fruit has the potential to bioaccumulate small organic molecules. Volatile organic compounds detected in the fruit pulp were absent from the processed commercial juice. Other benzenoid compounds ? thiazole and (+)-δ-cadinene, which are important biosynthetic precursors ? were identified from the juice sample. Molecular networking analysis of the tandem MS data of the ethanol extract of the juice putatively identified the presence of 1-kestose and sucrose. 1-Kestose is considered as an essential prebiotic compound associated with boosting metabolism and immunity. Other kestose isomers were also indicated to be present in the juice based on the elution profile and MS/MS data. Preliminary activity tested for both samples yielded positive result against Candida albicans using disc diffusion asay. Only the juice sample yielded significant activity against Escherichia coli. Chemistry. Calabash. Crescentia cujete l. Gc-ms. Lc-ms/ms. Metabolite profiling. Untargeted metabolomics. Dasmarinas, Dianne D. author. Junio, Hiyas A. author. The Philippine Journal of Science Vol. 147, no. 4, December 2018. Request full-text access via UPB University Library through https://forms.gle/KZjBv7aRtY6jiL5E9 (viewed 19 March 2021) FI UPBAG UPBAG-MAIN ARTICLE-2762 Analytics