00000caa a22000003i 4500 UP-1685594773862388489 Buklod 20210316141311.0 m | | ta 210316s2018 xx r |||| u|eng d (iLib)UPBAG-00039513679 STII-DOST BAG rda eng ARTICLE-2682 Juliadiningtyas, Ayu Dita author. Preliminary investigation of the carotenoid composition of erythrobacter sp. strain KJ5 by high-performance liquid chromatography and mass spectrometry by Ayu Dita Juliadiningtyas, Delianis Pringgenies, Heriyanto, Katarina Purnomo Salim, Ocky Karna Radjasa, Yuzo Shioi, Leenawaty Limantara, and Tatas Hardo Panintingjati Brotosudarmo. 2018. pages 91-98 color illustrations 26 cm text rdacontent unmediated rdamedia volume rdacarrier Includes bibliographical references (pages 96-98) Separation and identification of carotenoids (Cars) from aerobic marine bacterium Erythrobacter sp. strain KJ5 are reported. The cells of Erythrobacter sp. were grown in a Shioi medium at 28.5°C for three days. Among the four solvents tested, the mixture of methanol and acetone (3:7, v/v) was determined as the optimum solvent for Car extraction from the cells by measuring its absorption spectrum. The Cars were separated via reversed-phase high-performance liquid chromatography using a C8 column and identified by a UV-Vis photodiode array detector and an electrospray ionization mass spectrometry. Bacteriochlorophyll α was not detected from the extracts of cells grown under both light and dark conditions. At least 16 peaks of Cars were separated, wherein eleven peaks showed the same absorption spectrum with λmax at 452-453 nm and at 478-480 nm. The other five peaks had an additional absorption peak at 340 nm, which belongs to cis-isomeric form. Two peaks of Cars were identified to be zeaxanthin and β-carotene. Biology. #946-carotene. Carotenoids. Erythrobacter sp. High-performance liquid chromatography. Mass spectrometry. Zeaxanthin. Brotosudarmo, Tatas Hardo Panintingjati author. Limantara, Leenawaty author. Shioi, Yuzo , Radjasa author. Ocky Karna author. Salim, Katarina Purnomo author. Heriyanto author. Pringgenies, Delianis author. The Philippine Journal of Science Vol. 147, no. 1, March 2018. Request full-text access via UPB University Library through https://forms.gle/KZjBv7aRtY6jiL5E9 (viewed 16 March 2021) FI UPBAG UPBAG-MAIN ARTICLE-2682 Analytics