00000caa a22000003i 4500 UP-1685594773862388485 Buklod 20210316131222.0 m | | ta 210316s2018 xx r |||| u|eng d (iLib)UPBAG-00039513675 STII-DOST BAG rda eng ARTICLE-2678 Bascos, Neil Andrew D. author. Biophysical and functional characterization of asFP504, a novel fluorescent protein from the Philippines by Neil Andrew D. Bascos, Francine Lianne C. Emralino, Franco Carlos Liu, Carla P. Concepcion, Marvin Altamia, Yen-Chieh Huang, Yin-Cheng Hsieh, Chun-Jung Chen, and Cynthia Palmes-Saloma. 2018. pages 65-74 color illustrations 26 cm text rdacontent unmediated rdamedia volume rdacarrier Includes bibliographical references (pages 73-74) Fluorescent proteins have proven to be invaluable for a myriad of applications in scientific research. The discovery and characterization of novel fluorescent proteins promises to expand this range even further. This report focuses on the biophysical and functional characterization of a novel green fluorescent protein cloned from a Philippine soft coral species. The asFP504 protein showed peak excitation at 471 nm and at 494 nm (λE1= 471 nm; λE2=494 nm), its emission maximum from 471 nm excitation was observed at 504 nm. The fluorescence was observed to be related to its oligomeric state. Both fluorescence and oligomerization were robustly maintained for a range of temperatures, pH conditions, treatment with chaotropic agents, and proteolysis. X-ray crystallography documented a molecular packing of three dimers within each asymmetric unit for the asFP504 protein. The observed absorbance and fluorescence properties are comparable to that of commercially available fluorescence proteins. Despite its lower absorbance, asFP504 has higher quantum yield than mCitrine. In addition, the stability of asFP504 in the presence of multiple denaturants presents the potential of this protein â?? the first fluorescent protein from the Philippines-for use in many different research applications. Chemistry. Crystal structure. Fluorescent protein. Mutagenesis. Fluorescence resonance energy transfer (fret) Green fluorescent protein (gfp) Palmes-Saloma, Cynthia author. Chen, Chun-Jung author. Hsieh, Yin-Cheng author. Huang, Yen-Chieh author. Altamia, Marvin author. Concepcion, Carla P. author. Emralino, Francine Lianne C. author. Liu, Franco Carlos author. The Philippine Journal of Science Vol. 147, no. 1, March 2018. Request full-text access via UPB University Library through https://forms.gle/KZjBv7aRtY6jiL5E9 (viewed 16 March 2021) FI UPBAG UPBAG-MAIN ARTICLE-2678 Analytics