00000caa a22000003i 4500 UP-1685594773862388388 Buklod 20210311122622.0 m | | ta 210311s2017 xx r |||| u|eng d (iLib)UPBAG-00039458390 STII-DOST BAG rda eng ARTICLE-2590 Subong, Bryan John J. author. Toxicity and protein expression of Alexandrium species collected in the Philippine waters by Bryan John J. Subong, Garry A. Benico, Arielle Kae L. Sulit, Christopher O. Mendoza, Lourdes J. Cruz, Rhodora V. Azanza, and Elsie C. Jimenez. 2017. pages 425-436 illustrations (some color) 26 cm text rdacontent unmediated rdamedia volume rdacarrier Includes bibliographical references (pages 433-436) Isolates of Alexandrium species collected in the Philippine waters were examined during the exponential growth phase to compare their toxicities and protein expression profiles, and also to correlate protein expression with toxin production. Molecular methods showed that Alexandrium cf. pacificum was genetically divergent from Alexandrium tamarense complex Group IV/ Alexandrium pacificum. Toxin analyses using pre-oxidation method prior to HPLC purification were carried out to determine saxitoxin (STX), neosaxitoxin (neoSTX), and gonyautoxins 1-4 (GTX) levels. This study showed that cultured Alexandrium affine collected in Honda Bay, Palawan produced different STX analogs at various times of culture, which differed from other results showing that this species was non-toxic. The cultured Alexandrium cf. pacificum collected in Anda Channel (ATANDA) was two-fold more toxic than the cultured Alexandrium cf. pacificum collected in Bolinao Channel (ATBOL). Comparative protein expression analyses using 2-D gel electrophoresis were performed for the two Alexandrium cf. pacificum strains (ATANDA and ATBOL) during the exponential growth phase. A unique 2-DE protein spot in ATANDA showed sequence homology with bifunctional ornithine acetyltransferase/ N-acetylglutamate synthase (ArgJ) that has a role in the biosynthesis of arginine, a precursor in STX biosynthesis. The greater detectable expression of such enzyme in the ATANDA strain was correlated with the greater toxicity, suggesting the enzyme?s major participation in toxin biosynthesis. (Author's abstract) Marine species. Alexandrium sp. Harmful algal bloom. Paralytic shellfish poisoning. Paralytic shellfish. Toxin. Proteomics. Saxitoxin. Benico, Garry A. author. Sulit, Arielle Kae L. author. Mendoza, Christopher O. author. Cruz, Lourdes J. author. Azanza, Rhodora V. author. Jimenez, Elsie C. author. The Philippine Journal of Science Vol. 146, no. 4, December 2017. Request full-text access via UPB University Library through https://forms.gle/KZjBv7aRtY6jiL5E9 (viewed 11 March 2021) FI UPBAG UPBAG-MAIN ARTICLE-2590 Analytics