00000caa a22000003i 4500 UP-1685594773862388363 Buklod 20210310210125.0 m | | ta 210310s2017 xx r |||| u|eng d (iLib)UPBAG-00039458365 STII-DOST BAG rda eng ARTICLE-2568 Biendima, Cyndi C. author. Molecular characterization of BRCA1 as candidate gene marker for subclinical mastitis in Dairy water buffaloes (Bubalus bubalis) by Cyndi C. Biendima, Sonny C. Ramos, Mary Rose D. Uy, and Claro N. Mingala. 2017. pages 293-298 illustrations (some color) 26 cm text rdacontent unmediated rdamedia volume rdacarrier Includes bibliographical references (page 298) Subclinical mastitis (SCM), a major problem in dairy animals all over the world, is caused by intramammary infections with coagulase-negative staphylococci. It causes enormous losses for breeders since it suppresses the milk yield and quality; hence, it influences the national income of the country. It is related to low milk production, altered milk consistency, low protein content and high risk of contamination due to pathogenic organisms. The goal of the present study is to discover new information about the gene of interest, breast cancer 1 (BRCA1), early onset in the water buffalo genome by examining the presence of single nucleotide polymorphisms (SNPs) in water buffaloes. DNA from milk samples was isolated from thirty female riverinetype water buffaloes. Somatic cell score (SCS) was calculated from somatic cell count for each sample. The results showed two animals reached greater than 5, 5.6 and 5.8 SCS, respectively, which is indicative of SCM infection. Multiple sequence alignment revealed that all samples were monomorphic for BRCA1 gene. The genetic homology is undeniably high; however, it is not conclusive that the polymorphism found in cattle can also be found in water buffaloes. It is recommended that other single nucleotide polymorphisms in other exon should be studied. Also, the search for other candidate genes and larger sample size are essential to fully understand if these polymorphisms really affect the susceptibility of animals to SCM. (Author's abstract) Biology. Brca1 gene. Somatic cell. Subclinical mastitis. Water buffalo. Uy, Mary Rose D. author. Mingala, Claro N. author. The Philippine Journal of Science Vol. 146, no. 3, September 2017. Request full-text access via UPB University Library through https://forms.gle/KZjBv7aRtY6jiL5E9 (viewed 10 March 2021) FI UPBAG UPBAG-MAIN ARTICLE-2568 Analytics