00000caa a22000003i 4500 UP-1685594773862388221 Buklod 20210305112500.0 m | | ta 210305s1983 xx r |||| u|eng d (iLib)UPBAG-00039403008 STII-DOST BAG rda eng ARTICLE-2463 Pocsidio, Glorina N. author. Holothurin of some Philippine Holothurians and its hemolytic activity by Glorina N. Pocsidio. 1983. pages 13-28 illustrations 26 cm text rdacontent unmediated rdamedia volume rdacarrier Includes bibliographical references (pages 26-28) Four Philippine holothurian species namely Holothuria fuscocinerea Jaeger, Holothuria pulla Selenka, Actinophyga lecanora Jaeger, and Opheodesoma grisea semper were studied for their holothurin extraction yields, hemolytic potency of the toxin, and some nutrient contents after holothurin extraction. On dry weight basis, holothium yields were for H. fuscocinerea, 1.74% H. pulla, 1.64% A, lecanova, 1.24% and O. grisea. ).89%. On fresh weight basis, holothurin yields were for H. fuscocinera, 0-27%, H. pulla, 0.14%, A. lecabova, 0.10% O. grisea, 0.09%. The least concentrations at which hemolysis was observed with holothurin in 2% human red blood cell suspension were for A. lecanora, 0.060% mg/ml, H. pulla, 0.060 mg/ml H fuscocinera, 0.015 mg/ml and O. grisea, 4 mg/ml. Aqueous extract of the cuvierian tubules of H. pulla was active at tle least concentration of 1 mg/ml. The C50 values or concentrations for 50% hemolysis in undiluted human blood are: H. fuscocinerea, 0.15 mg/ml, H. pulla, 0.60% mg/ml and A. lecanora 0.71 mg/ml which gave the order of hemolytic potency as follows: H fuscocinerea> H pulla> A lecanora. The dried body walls after holothurin extraction contained protein in A. lecanora, the dried body walls after holothurin extraction contained protein A. lecanora of 11.0% H pulla 65.3% and H. fuscocinerea 45.6% and high levels of calcium, phosphorous and iron in three species. Zoology. Holothurin Hemolytic properties. Actinopyga lecanora. Holothurin Extraction. Sea cucumbers. The Philippine Journal of Science Vol. 112, nos. 1-2, January-June 1983. Request full-text access via UPB University Library through https://forms.gle/KZjBv7aRtY6jiL5E9 (viewed 05 March 2021) FI UPBAG UPBAG-MAIN ARTICLE-2463 Analytics