00000caa a22000003i 4500 UP-1685594773862388220 Buklod 20210305111533.0 m | | ta 210305s1983 xx r |||| u|eng d (iLib)UPBAG-00039403007 STII-DOST BAG rda eng ARTICLE-2462 Pocsidio, Glorina N. author. The mutagenicity potential of holothurin of some Philippine holothurians by Gloria N. Pocsidio. 1983. pages 1-12 illustrations 26 cm text rdacontent unmediated rdamedia volume rdacarrier Includes bibliographical references (pages 10-12) Holothurin in the form of ethanolic extract from the body walls of Actinopygalecanora Jaeger, Holothuria pulla Selenka and Holothuria fuscocinerea Jaeger was investigated for its mutagenicity potential. With the rec-assay on the Bacillus subtilisMarburg H17 (Rec) and M45 (Rec) strains, holothurin did not have direct DNA-damaging capacity, The Ames' test on Salmonella typhinuriumn strains TA1535 andTA1537 also demonstrated the infectivity of holothurin as a point mutagen before metabolic activation. However, holothurin was found highly mutagenic and clastogenicin vivo with the micronucleus test in the Swiss albino inice producing marked dose-related aberration scores as high as 62° / oo at 100 mg/kg H. fuscocinerea, 60°/00 at 200mg/kg H. pulla, and 51°/00 at 200 mg/kg A. lecanora administered intraperitoneally. Mutagenicity and clastogenicity of holothurin was reduced about 35-fold when administered orally. Toxic doses of holothurin adıninistered intraperitoneally to Swiss albino mice were at the minimal amounts of 200 mg/kg H. fu scocinerea and 700 mg/kgH. pulla. Biological Sciences. Holothurin Mutagenic properties. Actinopyga lecanora. Holothuria pulla. Holothuria fuscocinerea. The Philippine Journal of Science Vol. 112, nos. 1-2, January-June 1983. Request full-text access via UPB University Library through https://forms.gle/KZjBv7aRtY6jiL5E9 (viewed 05 March 2021) FI UPBAG UPBAG-MAIN ARTICLE-2462 Analytics