|
|
|
|
| LEADER |
03191ctm a22003493a 4500 |
| 001 |
UP-1685594773861923532 |
| 003 |
Buklod |
| 005 |
20130511084346.0 |
| 006 |
g||| | || || |
| 007 |
ta |
| 008 |
200523s2013 xx ab rbm |||| u|eng d |
| 035 |
|
|
|a (iLib)UPBAG-00010217757
|
| 040 |
|
|
|a BAG
|e rda
|
| 041 |
0 |
|
|a eng
|
| 090 |
|
0 |
|a Thesis
|
| 100 |
1 |
|
|a Alvarado, Berlie A.
|e author.
|
| 245 |
1 |
0 |
|a Archaeal and bacterial community analysis of Mainit Hot Spring, Bontoc, Mt. Province
|c by Berlie A. Alvarado, Arian U. Balolong, Maria Dawn Rosary B. Yap.
|
| 264 |
|
1 |
|c 2013.
|
| 300 |
|
|
|a xii, 185, [27] leaves
|b illustrations (some color), color map
|c 29 cm+
|e 1 computer optical disc (4 3/4 in.)
|
| 336 |
|
|
|a text
|2 rdacontent
|
| 337 |
|
|
|a unmediated
|2 rdamedia
|
| 338 |
|
|
|a volume
|2 rdacarrier
|
| 502 |
|
|
|a Thesis (Bachelor of Science) -- University of the Philippines Baguio, 2013.
|
| 504 |
|
|
|a Includes bibliographical references (leaves [189-112])
|
| 520 |
|
|
|a Hot springs support many hyper- and thermophilic prokaryotic communities but the overall community diversity of bacteria in unexplored thermophilic environments is still poorly understood. A sequence- based survey of bacterial communities present in sediment samples derived from Mainit Hot Spring in Bontoc, Mountain Province was undertaken using the Roche 454 shotgun sequencing method. Analyses of the community 16S rRNA using Metagenomics-RAST (MG-RAST) server and Metagenome Analyzer (MEGAN) revealed that the Phyla Aquificae, Crenarchaeota, Deinooccus-Thermus, Firmicutes and Proteobacteria were the most abundant. The dominant genera identified were Acinetobacter, Bacillus, Hydrogenobacter, Pyrobaculum, and Thermus. Sequence analysis also revealed that there are bacterial species which may be possible source of the enzyme DNA polymerase used in the process of Polymerase Chain Reaction (PCR) and this were the following: Pyrococcus furiosus, Pyrobaculum islandicum and Methanothermobacter thermoautotrophicus for archea and the Thermus aquaticus for bacteria. Mainit hot spring has a temperature of more than 100 degrees C, has a soil pH of 8.38 and is relatively high in calcium (Ca2+), potassium (k+), dissolved oxygen (DO), chloride (CI2), sulphate (SO2- 4) and nitrate nitrogen. These physico-chemical properties of the hot spring could possibly have some influence on the diversity of archea and bacteria. This study reports the first metagenome analysis of the archaeal and bacterial communities in a Cordilleran thermal spring. The results of this study can therefore serve as baseline information that can contribute to the understanding of hyper- and thermophilic microbial communities in the Cordilleras and can provide a basis for comparison with other geothermal systems in other parts of the Philippines and the world -- Author's abstract.
|
| 650 |
|
0 |
|a Archaeabacteria
|z Philippines
|z Mainit (Bontoc, Mountain Province)
|x Identification.
|
| 650 |
|
0 |
|a Bacteria
|z Philippines
|z Mainit (Bontoc, Mountain Province)
|x Identification.
|
| 700 |
1 |
|
|a Balolong, Arian U.
|e author.
|
| 700 |
1 |
|
|a Yap, Maria Dawn Rosary B.
|e author.
|
| 905 |
|
|
|a UP
|
| 852 |
1 |
|
|a UPBAG
|b UPBAG-MAIN
|h Thesis
|
| 942 |
|
|
|a Thesis
|