00000ctm a22000003a 4500 UP-1685523046126355576 Buklod 20230302143710.0 m |o d | cr ||||||||||| 230302s2019 xx d r |||| u| (iLib)UPMNL-00017456973 UPDent rda eng LG993.5 2019 D4 B37 Bartolome, Thea Liza DG author The antimicrobial efficacy of crude extract of carmona retusa (Vahl) masam leaves on the inhibition of streptococcus mutans-an in vitro study Thea Liza DG. Bartolome, Frances Nicole T. Chan, Paolo Romeo V. Dorado and Janella Ivane T. Lacson. Manila Department of Community Dentistry, University of the Philippines Manila c2019. 89 leaves color illustrations 28 cm. text rdacontent unmediated rdamedia volume rdacarrier Seminar Paper--Doctor of Dental Medicine--University of the Philippines Manila Yes No No INTRODUCTION: A study on Carmona retusa, commonly known as Wild Tea or Tsaang gubat, has demonstrated an anticariogenic effect due to its fluoride content. Part of the normal flora in the human oral cavity, Streptococcus mutans is known to play a major role in the etiology of dental caries. In this study, crude extract of dried Carmona retusa leaves was evaluated for its antimicrobial efficacy against Streptococcus mutans, in vitro. METHODS: Crude extract concentrations (25%, 50%, and 100%) were prepared from dried and powdered Carmona retusa leaves with water as the solvent. The antimicrobial efficacy of the prepared concentrations was tested using the agar disk diffusion method. The zone of inhibitions produced by the Carmona retusa crude extract of different concentrations were determined after 24 hours of incubation at 35°C. These were measured using a 6-inch ruler and compared to the positive control (0.12% Chlorhexidine digluconate) and the negative control (sterile distilled water). RESULTS: The mean zones of inhibition produced by 0.12% chlorhexidine was 24.23 mm while the negative control and various concentrations of the Carmona retusa crude extract did not inhibit bacterial growth?0mm mean diameter of zones of inhibition. CONCLUSION: Carmona retusa leaves aqueous crude extract did not exhibit antimicrobial efficacy against the growth of Streptococcus mutans and therefore, may not be effective. Streptococcus mutans. Carmona retusa. Chan, Frances Nicole T. author. Dorado, Paolo Romeo V. author. Lacson, Janella Ivane author. UPMNL DENT LG993.5 2019 D4 B37 Thesis