00000ctm a22000003a 4500 UP-1685523046126318406 Buklod 20170810165433.0 m |o d | ta 170810s2017 xx d r |||| u| (iLib)UPMNL-00015498123 UPM PHARM rda eng LG 993.5 2017 P5 B33 Badong, Ronnie Antonio G. author. Influence of environmental conditions to α-glucosidase inhibitory activity of Caimito (Chrysophyllum cainito) leaves Ronnie Antonio G. Badong, Michael John C. Rivera, Vivente Paolo A. Rubio ; adviser, Joanna V. Toralba. ​​​​​​​​​​Manila: Department of Pharmacy, College of Pharmacy, University of the Philippines Manila 2017. 65 leaves color illustrations 29 cm. ​​​​text rdacontent unmediated rdamedia volume rdacarrier Available to the general public. ​​​​​​Thesis (Bachelor of Science in Pharmacy)--University of the Philippines Manila, June 2017. This study can be used as a reference for characterization of C. cainito leaves with respect to their phytochemical profile and determine when and where the ideal season of collection is. This study aims to evaluate the influence of location and/or season of collection of C. cainito leaves on the TLC profile and its alpha-glucosidase inhibitory property. The samples were collected from 6 regions of the Philippines: Region II, Region III, Region IV, NCR, Region VII and Region IX in the months of November 2016 and March 2017. The samples were extracted and partitioned separately using modified Kupchan partitioning. Only the ethyl acetate fraction was used in the test for phytochemicals specifically for anthraquinones, tannins and phenolic glycoside, of the three only the ferric chloride test for the presence of phenolic glycosides and tannins had a positive result. TLC was performed on the ethyl acetate fractions of all the samples including the anthraquinone standard. The results suggest that C. cainito leaves from the six different regions and two different seasons of harvest have the same chromatographic profile. Anthraquinones are also observed to be present in all of the sample excluding the obscured spots in Regions NCR and VII from the dry season. The ethyl acetate fraction of each sample was evaporated to dryness, dissolved in methanol and was tested for their inhibitory property in three different concentrations (30 μg/mL, 70 μg/mL, 150 μg/mL) in a 96-well plate bioassay. The results of the bioassay suggest that the location and/or season of collection do not affect the inhibitory property of C. cainito leaves. Environmental conditions. Chrysophyllum cainito. Plant extracts. Rivera, Michael John C. author. Rubio, Vivente Paolo A. II author. Toralba, Joanna V. thesis adviser. FI UP UPMNL PHARM LG 993.5 2017 P5 B33 Thesis