00000ctm a22000003a 4500 UP-1685523046126318405 Buklod 20170810162138.0 m |o d | ta 170810s2017 xx d r |||| u| (iLib)UPMNL-00015498122 UPM PHARM rda eng LG 993.5 2017 P5 B35 Balutoc, Iris Patrisha Abigail C. author. Beta-lactamase inhibition activity screening of methanolic and ethanolic extracts of selected Philippine plants Iris Patrisha Abigail C. Balutoc, Vanna Melinda S. Calimon, Daryl E. Magno ; adviser, Joanna V. Toralba. ​​​​​​​​​​Manila: Department of Pharmaceutical Chemistry, College of Pharmacy, University of the Philippines Manila 2017. vii, 105 leaves color illustrations 29 cm. ​​​​text rdacontent unmediated rdamedia volume rdacarrier Available to the general public. ​​​​​​Thesis (Bachelor of Science in Industrial Pharmacy)--University of the Philippines Manila, June 2017. The discovery of antibiotics resulted to a therapeutic revolution which gave cure to lethal infectious diseases and disabled infections. However, the emergence of resistance to β-Lactam antibiotics rendered the most widely produced and used antibacterial drugs in the world useless. This study generally aims to evaluate the β-lactamase inhibition activity of selected Philippine plant samples. The β-lactamase inhibitory action of methanolic or ethanolic extracts of fifty plant samples were quantified through nitrocefin competition assay. The extracts of Durio zibethinus, Voacanga globosa, Moringa oleifera, and Melanolepis multiglandulosa showed 70.05%, 80.18%, 68.39%, and 52.97%, respectively. Thin-layer chromatography and phytochemical constituent testing were done to characterize the extracts. The methods, however, did not yield conclusive results which can be attributed to the low concentration of phytochemical constituents present in the extract. Since the susceptibility and synergistic effects of the four plant samples was not determined, the four samples can be tested to clinical isolates to determine its activity against microorganism with beta-lactamase enzyme. Plant extracts. Calimon, Vanna Melinda S. author. Magno, Daryl E. author. Toralba, Joanna V. thesis adviser. FI UP UPMNL PHARM LG 993.5 2017 P5 B35 Thesis