00000ctm a22000003a 4500 UP-1685523046126268359 Buklod 20140508151220.0 m |o d | ta 140508s xx d r |||| u| (iLib)UPMNL-00013079728 UPM Pharm eng LG993.5 2014 P5 O25 Obispo, Krishelle Anne M. Molluscicidal activity and bioassay-guided fractionation of crude extracts of Annona squamosa L. (Fam. Annonaceae) against oncomelania quadrasi Krishelle Anne M. Obispo, Ihris Jay b. Terrado, Ma. Krystelle Marianne C. Yap. viii,45 leaves The seed, leaf, and bark of Annona squamosa L. (Fam. Annonaceae) were screened for molluscicidal activity, using the molluscicidal bioassay against Oncomelania quadrasi. The crude aqueous extract of the bark among the six extracts, exhibited molluscicidal activity at the concentration of 0.1 g/L. The % mortality of the O. quadrasi showed an increasing trend as the concentration of the crude aqueous bark extract is increased. The crude aqueous bark extract was subjected to bioassay-guided fractionation using modified Kupchan Partitioning resulting to aqueous, hexane, ethyl acetate, and methanol fractions. Of the four solvent fractions, the aqueous fraction showed the highest molluscicidal activity with % mortality reaching up to 50%. Tannins, flavonoids and terpenoids were found in the aqueous fraction of the crude aqueous bark extract, which may be the responsible compound that contributed to its molluscicidal activity. The results suggest that A. squamosa may be used as a potential molluscicidal agent against O.quadrasi for the eradication of schistosomiasis. Molluscicides. Terrado, Ihris Jay B. Yap, Ma. Krystelle Marianne C. UPMNL PHARM LG993.5 2014 P5 O25 Thesis