Optimization of a high performance liquid chromatography method with solid phase extraction to quantify quercetin from the leaves of Blumea balsamifera (L) DC (Family Asteraceae)
The quality control of Department of Health approved herbal medicines currently includes thin layer chromatography and crude drug analyses of the raw materials and can be augmented by developing quantitative analysis of specific compounds found in the plant material. This tudy was undertaken as an...
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| Resource Type: | Thesis |
| Language: | English |
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| Summary: | The quality control of Department of Health approved herbal medicines currently includes thin layer chromatography and crude drug analyses of the raw materials and can be augmented by developing quantitative analysis of specific compounds found in the plant material. This tudy was undertaken as an attempt to develop a High Performance Liquid Chromatography (HPLC) method with solid phase extraction (SPE) for the quantitation of Quercetin in the leaves of Sambong (Blumea balsamifera (L) DC) from Leyte, Cotabato and Nueva Ecija. The methanol extracts of Sambong were prepared by maceration followed by rotary evaporation. The development of the SPE involved the investigation of the sample preparation and loading, washing solvent, and eluting solvent. The optimum conditions for the SPE involved the use of octadecyl silane SPE packing, a 0.5-mL sample load (50 mg/mL solution), and elution with 4-mL of 80:20 Methanol-0.5% H3PO4. The HPLC method development included the determination of the wavelength of detection, flow rate of mobile phase, mode of elution, and mobile phase composition for gradient elution. The optimum conditions for the HPLC determination of quercetin involved the use of octadecyl silane stationary phase in a 4.6mm x 250mm column and maintained at 30°C, 254-nm wavelength of detection, 1 mL/min mobile phase flow rate, and a mobile phase composition of methanol, 0.5% H3PO4, and 0.2% triethylamine in gradient elution. The validation studies resulted to a good linearity of r2=0.9989 at the concentration range of 3.72 ? 124 µg/mL of Quercetin standard, a limit of detection of 0.684 µg/mL and limit of quantitation of 2.280 µg/mL, accuracy of 102.41%, and precision values of less than 2%. The quercetin contents determined are 0.2337mg, 0.1350mg, and 0.2940mg per gram of the powdered leaves of Sambong from Nueva Ecija, Cotabato, and Leyte respectively. |
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| Item Description: | Thesis (Master of Science in Pharmaceutical Chemistry)--University of the Philippines Manila, 2012. Available to the general public. |
| Physical Description: | xix, 116 leaves |